Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 1999 | Volume: 2 | Issue: 4 | Page No.: 1299-1304
DOI: 10.3923/pjbs.1999.1299.1304
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Purification and Characterization of New Type II Restriction Endonucleases

Shahnaz Choudhry

Abstract:
Twelve bacterial strains were screened for the presence of new type II restriction endonucleases. Two are reported positive for the enzyme activity, and designated as BthDI, SarI, from Bacillus thuringiensis D4 (CAMB 2657) and Streptomyces aureomonopodiales (ATCC 19825). These enzymes have been purified by a combination of gel filtration, ion exchange and affinity chromatography. The DNA sequences recognized by these enzymes have been determined by analysis of the fragment patterns generated on different substrate DNAs, Lambda, T7, pUC19, pBR322, PhiX174RFI, and Adenovirus-2 (Ad-2). The cleavage sites within the recognition sequences have also been established by primed synthesis. Accordingly, the newly discovered enzymes BthDI and SarI recognize and cleave 4-6 nucleotide long stretch of DNA, CC!AGG, AGG! CCT, respectively.
PDF Citation Report Citation
How to cite this article:

Shahnaz Choudhry , 1999. Purification and Characterization of New Type II Restriction Endonucleases . Pakistan Journal of Biological Sciences, 2: 1299-1304.

DOI: 10.3923/pjbs.1999.1299.1304

URL: https://scialert.net/abstract/?doi=pjbs.1999.1299.1304

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom