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Pakistan Journal of Biological Sciences
  Year: 1998 | Volume: 1 | Issue: 4 | Page No.: 386-388
DOI: 10.3923/pjbs.1998.386.388
 
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Isolation and Characterization of Acid Phosphatase from Chickpea Seeds (Cicer arietinum)

Razia Khan, Mohammad Tariq, Anwar Khan, Naheeda and S.F. Mabood

Abstract:
Acid phosphatase (AcP., EC: 3.1.3.2) from chickpea powder was extracted with acetate buffer. Best extraction was carried out at pH 5.5. The enzyme was found to be more stable at pH 5.5 at 4°C for a period of one month incubation. The activity of the extract stored in vitro at 4°C was quite stable for upto 1 month. The enzyme had an optimum pH 5.5 and optimum temperature of 30°C. The Km value was found to be 8.3, μm for p-nitrophenyl phosphate as substrate.
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How to cite this article:

Razia Khan, Mohammad Tariq, Anwar Khan, Naheeda and S.F. Mabood, 1998. Isolation and Characterization of Acid Phosphatase from Chickpea Seeds (Cicer arietinum). Pakistan Journal of Biological Sciences, 1: 386-388.

DOI: 10.3923/pjbs.1998.386.388

URL: https://scialert.net/abstract/?doi=pjbs.1998.386.388

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