
	Pakistan Journal of Biological Sciences
	Year: 1998 \| Volume: 1 \| Issue: 4 \| Page No.: 366-367 DOI: 10.3923/pjbs.1998.366.367

Isolation and Characterization of Acid Phosphatase from Wheat (Pirsabak-85) Grains (Triticum aestivum)

Naheed Ahmad Shah, Razia Khan, Mohammad Tariq and S.F. Mabood

Abstract: Acid phosphatase (Acp., 3.1.3.2) from wheat grain powder was extracted with acetate buffer. Maximum extraction was carried out at pH 5.5. The activity of the extract stored in vitro at 4°C was quite stable for upto one week. The enzyme had optimum pH 5.5 and optimum temperature of 30°C. The K_m value was found to be 10 μM for P-introphenyl phosphate as a substrate.

PDF Fulltext XML References Citation Report Citation

How to cite this article:

Naheed Ahmad Shah, Razia Khan, Mohammad Tariq and S.F. Mabood, 1998. Isolation and Characterization of Acid Phosphatase from Wheat (Pirsabak-85) Grains (Triticum aestivum). Pakistan Journal of Biological Sciences, 1: 366-367.

DOI: 10.3923/pjbs.1998.366.367

URL: https://scialert.net/abstract/?doi=pjbs.1998.366.367

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		ISI Web of Knowledge
		PubMed
		SCIMAGO
		Thomson ISI

Science Alert