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Pakistan Journal of Biological Sciences
  Year: 1998 | Volume: 1 | Issue: 4 | Page No.: 366-367
DOI: 10.3923/pjbs.1998.366.367
 
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Isolation and Characterization of Acid Phosphatase from Wheat (Pirsabak-85) Grains (Triticum aestivum)

Naheed Ahmad Shah, Razia Khan, Mohammad Tariq and S.F. Mabood

Abstract:
Acid phosphatase (Acp., 3.1.3.2) from wheat grain powder was extracted with acetate buffer. Maximum extraction was carried out at pH 5.5. The activity of the extract stored in vitro at 4°C was quite stable for upto one week. The enzyme had optimum pH 5.5 and optimum temperature of 30°C. The Km value was found to be 10 μM for P-introphenyl phosphate as a substrate.
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How to cite this article:

Naheed Ahmad Shah, Razia Khan, Mohammad Tariq and S.F. Mabood, 1998. Isolation and Characterization of Acid Phosphatase from Wheat (Pirsabak-85) Grains (Triticum aestivum). Pakistan Journal of Biological Sciences, 1: 366-367.

DOI: 10.3923/pjbs.1998.366.367

URL: https://scialert.net/abstract/?doi=pjbs.1998.366.367

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