N. Altaf
Nuclear Institute for Agriculture and Biology, Jhang Road, Faisalabad
J. Iqbal
Nuclear Institute for Agriculture and Biology, Jhang Road, Faisalabad
Botany Department, Punjab University, Quaid-e-Azam Campus, Lahore-Pakistam
M. S. Ahmad
Nuclear Institute for Agriculture and Biology, Jhang Road, Faisalabad
Among the sugars tested, sucrose was the best for callussing. Auxin 2,4-D induced callussing but cytokinin enhanced formationof shoot primordia in explants. Both BA and K promoted multiple shoot formation but K and GA gave more shoot buds. Cytokinins with auxins gave callusing. Callus was also induced in explants when grown in K (10 mg L1) + GA (1 mg L1) containing medium in the dark. When callus was shifted to light it started regeneration as green dots. The addition of seed extract to K and GA medium considerably enhanced regenerations.
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How to cite this article
N. Altaf, J. Iqbal and M. S. Ahmad, 1998. Influence of Explant Source and Medium Ingredients on
Culture Responses of Microsperma Lentil Cultivar Masoor-85. Pakistan Journal of Biological Sciences, 1: 321-325.
DOI: 10.3923/pjbs.1998.321.325
URL: https://scialert.net/abstract/?doi=pjbs.1998.321.325
DOI: 10.3923/pjbs.1998.321.325
URL: https://scialert.net/abstract/?doi=pjbs.1998.321.325