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Pakistan Journal of Biological Sciences
  Year: 1998 | Volume: 1 | Issue: 4 | Page No.: 303-306
DOI: 10.3923/pjbs.1998.303.306
 
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Further Serological Characterization of Two Tobravirus Isolates from Algeria and Libya

K.M. Makkouk and S.G. Kumari

Abstract:
Antisera against purified particles of tobravirus isolates from Libya (LYV66-91) and Algeria (AlgRl 0) were produced and used for their serological comparison with other isolates of pea early-browning tobravirus (PEBV), including one of the broad bean yellow band serotypes (PEBV:BBYB) and with the PRN strain of tobacco rattle tobravirus (TRV:PRN). DASELISA, DAC-ELISA and dot-blot BLISA showed that the Algerian and the Libyan isolates represent two different serotypes. In DAS-BLISA, the Libyan isolate was similar to the Dutch isolate of PEBV (isolate El 16). In DAC-ELISA, the Algerian isolate reacted strongly with PEBV:BBYB and the TRV:PRN antibodies but not with those to the Libyan or the Dutch isolates of PBBV. Using dot-blot ELISA, the Libyan isolate was serologically closely related to the Dutch isolate of PBBV and distinct from the Algerian isolate of PEBV, PEBV:BBYB and TRV.PRN.
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How to cite this article:

K.M. Makkouk and S.G. Kumari, 1998. Further Serological Characterization of Two Tobravirus Isolates from Algeria and Libya. Pakistan Journal of Biological Sciences, 1: 303-306.

DOI: 10.3923/pjbs.1998.303.306

URL: https://scialert.net/abstract/?doi=pjbs.1998.303.306

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