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Pharmacologia

Year: 2014 | Volume: 5 | Issue: 6 | Page No.: 199-204
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Authors

Foutse Yimta

Mouokeu Raymond Simplice

Nguimatsia Francois

Njateng Guy Sedar Singor

Tamokou Jean de Dieu

Kuiate Jules Roger

Keywords

Background: Alternatives to available antibiotics for disease management are increasingly felt due to the increase in the resistance of bacterial strain. Plants are known to be a rich source of medicines because they produce wide array of bioactive molecules. The present study was undertaken to investigate the antibacterial properties of the methanol extract bark and leaves of Kalanchoe crenata (Crassulaceae), Terminalia avicennioides (Combretaceae) and Sarcocephalus latifolius (Rubiaceae). Materials and Methods: The crude extracts were prepared by maceration of plant powder in methanol. K. crenata extract was further partitioned into hexane, ethyl acetate and residue fractions. T. avicennioides extract was also fractionated by flash chromatography into eight fractions named Fc to Fj. Phytochemical tests were carried out on the extracts and fractions using standard methods. The antibacterial activity of the crude extracts and fractions were evaluated by broth microdilution method. Results: The phytochemical tests indicate that all tested extracts contained phenols, tannins, flavonoids and other classes of chemicals were selectively present. The antibacterial susceptibility test showed the best spectra of activity with T. avicennioides extract (MIC = 0.1-0.4 mg mL-1), followed by S. latifolius (MIC= 0.2-0.8 mg mL-1). K. crenata extract was found to be less active (MIC= 0.8-1.6 mg mL-1). FI and FJ fractions were found to have similar antibacterial activity relatively greater than FG fraction. The highest activity of those fractions was achieved on P. mirabilis (MIC =0.02 mg mL-1) where conventional antibiotic ciprofloxacin failed to be active. Conclusion: The overall results highlighted the antibacterial activity of T. avicennioides and S. latifolius. This constitutes a power tool for the investigation of T. avicennioides and S. latifolius extract for the preparation of phytomedicine against bacterial diseases as well as the isolation of active ingredient from T. avicennioides methanol extract.
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How to cite this article

Foutse Yimta, Mouokeu Raymond Simplice, Nguimatsia Francois, Njateng Guy Sedar Singor, Tamokou Jean de Dieu and Kuiate Jules Roger, 2014. Antibacterial Activity of Methanol Extracts and Fractions from Kalanchoe crenata, Terminalia avicennioides and Sarcocephalus latifolius. Pharmacologia, 5: 199-204.

URL: https://scialert.net/abstract/?doi=pharmacologia.2014.199.204

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