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Pharmacologia

Year: 2013 | Volume: 4 | Issue: 2 | Page No.: 95-106
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Authors

D.D. Bandawane

K.H. Bibave

A.V. Jaydeokar

U.S. Patil

M.G. Hivrale

Keywords

Research Article

Antihyperglycemic and Antihyperlipidemic Effects of Methanolic Extract of Holarrhena antidysenterica Bark in Alloxan Induced Diabetes Mellitus in Rats

D.D. Bandawane, K.H. Bibave, A.V. Jaydeokar, U.S. Patil and M.G. Hivrale
Background: Holarrhena antidysenterica L. (family Apocynaceae) is traditionally used in Ayurvedic system of Indian medicine for the treatment of diabetes mellitus. Therefore, the present work was undertaken to evaluate the antidiabetic and antihyperlipidemic effects of methanolic extract of Holarrhena antidysenterica Bark (MEHA) in alloxan induced diabetes mellitus and to focus on its possible mechanism. Materials and methods: Wistar albino rats (150-220 g) of either sex were used for the study. Diabetes was induced in rats by injecting alloxan (150 mg kg-1) intraperitoneally. Group I served as normoglycemic rats. Group II served as diabetic control. Group III and IV served as diabetic rats treated with 200 and 400 mg kg-1 of MEHA, respectively. Group V served as diabetic rats treated with oral hypoglycaemic agent, glibenclamide (4 mg kg-1 p.o.). Group VI and VII served as normoglycemic rats treated with 200 and 400 mg kg-1 of MEHA. All the treatments were given for 28 days. At the end of study, on 28th day, overnight fasted rats were sacrificed and blood was collected to determine fasting blood glucose, triglycerides, total cholesterol, high density lipoprotein, low density lipoprotein, very low density lipoprotein, total protein, blood urea nitrogen, alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase. To study in vivo antioxidant activity, liver tissues of different groups were homogenized to determine maloaldehyde (MDA), catalase (CAT), Super Oxide Dismutase (SOD) and reduced glutathione (GSH). Additional parameters were estimated to focus on mechanism of action were liver glycogen content and glucose uptake from hemidiaphragms. Results: Diabetic rats treated with MEHA in doses of 200 and 400 mg kg-1 significantly (p<0.01) reduced fasting blood glucose and normalized the lipid profile in comparison to diabetic control group. There was dose dependent decrease observed in transaminases, BUN and MDA whereas there was significant (p<0.01) improvement in total proteins, liver catalase, SOD and GSH in MEHA treated groups. MEHA (200 and 400 mg kg-1) treated diabetic rats showed significant improvement in liver glycogen and glucose uptake by rat diaphragm. Improvement in histopathology of pancreas of MEHA treated rats confirmed its protective role in alloxan induced diabetes. Conclusion: It can be concluded that MEHA possesses antihyperglycemic activity with antihyperlipidemic and antioxidant potential which may prove beneficial in cardiovascular complications associated with diabetes mellitus.
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D.D. Bandawane, K.H. Bibave, A.V. Jaydeokar, U.S. Patil and M.G. Hivrale, 2013. Antihyperglycemic and Antihyperlipidemic Effects of Methanolic Extract of Holarrhena antidysenterica Bark in Alloxan Induced Diabetes Mellitus in Rats. Pharmacologia, 4: 95-106.

URL: https://scialert.net/abstract/?doi=pharmacologia.2013.95.106

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