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Pharmacologia
  Year: 2013 | Volume: 4 | Issue: 1 | Page No.: 38-47
DOI: 10.5567/pharmacologia.2013.38.47
Immunomodulatory Activity of Ethyl Acetate Extract and Fractions from Leaves of Crassocephalum bauchiense (Asteraceae)
Raymond S. Mouokeu, Rosalie A.N. Ngono, Christopher Tume, Monique O. Kamtchueng, Guy S.S. Njateng, Dzoyem Jean Paul, Jean De Dieu Tamokou and Jules-Roger Kuiate

Abstract:
Medicinal plants are rich sources of secondary metabolites which are claimed to possess immunomodulatory properties, justifying why the present study was designed to investigate the immunomodulatory potential of Crassocephalum bauchiense leaves extracts. Leaves were macerated successively in hexane, ethyl acetate and methanol. The ethyl acetate extract was further fractionated into six fractions (F1 to F6) using flash chromatography. The activities of the extract and its fractions were investigated on macrophage NO production model while their proliferative effects were evaluated on macrophages and peripheral blood lymphocytes by the thiazolyl blue (MTT) test. Extract and fractions significantly inhibited NO production by peritoneal macrophages. However, they enhanced macrophage and lymphocyte proliferation in vitro at lower concentrations (<64 or <128 μg mL-1). Fraction F2 significantly increased Delayed-type herpersensitivity reaction (DTH) in mice. This activity was potentiated when the fraction was co-administered with cyclophosphamide. The fraction did not show any activity on antibody production. The total leucocytes count (mainly lymphocytes) was higher in the extract-treated groups when compared to the controls. Fraction F2 significantly suppressed myelosuppression induced by cyclophosphamide as from 25 mg kg-1 of body weight (bw). Oral administration of the fraction caused lethal signs of toxicity from 16 mg kg-1 bw. The LD50 was estimated at 22 mg kg-1 bw. These results indicate that C. bauchiense ethyl acetate extract and its fraction F2 have relatively interesting immunostimulatory effects on lymphocytes. In associated with its kwon antimicrobial properties, one can conclude that this plant species is a candidate for anti-infective phytomedicine preparation.
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How to cite this article:

Raymond S. Mouokeu, Rosalie A.N. Ngono, Christopher Tume, Monique O. Kamtchueng, Guy S.S. Njateng, Dzoyem Jean Paul, Jean De Dieu Tamokou and Jules-Roger Kuiate, 2013. Immunomodulatory Activity of Ethyl Acetate Extract and Fractions from Leaves of Crassocephalum bauchiense (Asteraceae). Pharmacologia, 4: 38-47.

DOI: 10.5567/pharmacologia.2013.38.47

URL: https://scialert.net/abstract/?doi=pharmacologia.2013.38.47

 
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