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Journal of Pharmacology and Toxicology

Year: 2023 | Volume: 18 | Issue: 3 | Page No.: 104-111
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Authors

Okafor Collins Onyebuchi Okeke

Okafor Collins Onyebuchi Okeke

Ude Ibiam Ude

Ude Ibiam Ude

Ovia Kenneth Ndidi

Eromonsele Blessing Osose

Oduokpaha Godwin Edet

Adie Upekiema Francisca

Obasi David Chukwu

Obasi David Chukwu

Ani Peace

Ani Peace

Ezeme-Nwafor Amarachukwu Chioma

Nwosu Mariagoretti Chimezie

Ezeme Udoakuchinyere Chimamanda

Iroha Ifeanyichukwu Romanus

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Research Article

Antibiotic Resistance Studies of Pseudomonas aeruginosa Harbouring blaCTX-M-1-CTXM-82 and blaIMP-1/IMP-2 Encoding Genes from a Water Treatment Reservoir in South Eastern Nigeria

Okafor Collins Onyebuchi Okeke Okafor Collins Onyebuchi Okeke's LiveDNA, Ude Ibiam Ude Ude Ibiam Ude's LiveDNA, Ovia Kenneth Ndidi, Eromonsele Blessing Osose, Oduokpaha Godwin Edet, Adie Upekiema Francisca, Obasi David Chukwu Obasi David Chukwu's LiveDNA, Ani Peace Ani Peace's LiveDNA, Ezeme-Nwafor Amarachukwu Chioma, Nwosu Mariagoretti Chimezie, Ezeme Udoakuchinyere Chimamanda and Iroha Ifeanyichukwu Romanus
Background and Objective: The detection of ARGs in drinking water systems in Nigeria is a serious wake-up call on the need for regular surveillance and control measures. This study was carried out to determine the presence of antibiotic-resistance genes in Pseudomonas aeruginosa isolated from the Water Treatment Reservoir in Ebonyi State, Nigeria. Materials and Methods: A total of 70 samples of water were examined in this study. Bacteriological analysis of the test isolates was carried out using standard microbiology techniques. Antibiotic susceptibility studies of the test isolates were determined using the Kirby-Bauer disc diffusion method. Isolates obtained were further identified and characterized by PCR analysis using 16S rRNA gene amplification. Results: A total of 56 (80%) isolates of Pseudomonas spp., were confirmed from 70 water samples tested. The isolates were resistant to imipenem (39.29%), ceftazidime (67.86%), ciprofloxacin (69.64%), ofloxacin (71.43%), ertapenem (83.93%) cefepime (85.71%), ticarcillin (89.29%), amikacin (89.29%), gentamicin (91.93%) and cefotaxime (92.86%) while showing complete resistant to amoxicillin\clavulanic acid, tobramycin, oxacillin and aztreonam (100%). The PCR analysis revealed that blaCTX-M-1-CTX-M-82 was present in Pseudomonas aeruginosa strain F18 16S, Pseudomonas tolaasii strain ATCC 33618 16S, Pseudomonas mendocina strain Y20 16S and Pseudomonas aeruginosa strain NA114 16S while blaIMP-1/IMP-2 gene was present only in Pseudomonas aeruginosa strain F18 16S and Pseudomonas aeruginosa strain NA114 16S. Conclusion: We, report the occurrence of multidrug-resistant Pseudomonas spp., harbouring Extended-Spectrum Beta-Lactamase (ESBL) and carbapenemase resistance genes from a water treatment reservoir in Nigeria.
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Okafor Collins Onyebuchi Okeke, Ude Ibiam Ude, Ovia Kenneth Ndidi, Eromonsele Blessing Osose, Oduokpaha Godwin Edet, Adie Upekiema Francisca, Obasi David Chukwu, Ani Peace, Ezeme-Nwafor Amarachukwu Chioma, Nwosu Mariagoretti Chimezie, Ezeme Udoakuchinyere Chimamanda and Iroha Ifeanyichukwu Romanus, 2023. Antibiotic Resistance Studies of Pseudomonas aeruginosa Harbouring blaCTX-M-1-CTXM-82 and blaIMP-1/IMP-2 Encoding Genes from a Water Treatment Reservoir in South Eastern Nigeria. Journal of Pharmacology and Toxicology, 18: 104-111.

DOI: 10.3923/jpt.2023.104.111

URL: https://scialert.net/abstract/?doi=jpt.2023.104.111

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