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Journal of Pharmacology and Toxicology
  Year: 2020 | Volume: 15 | Issue: 2 | Page No.: 58-64
DOI: 10.3923/jpt.2020.58.64
 
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Sedative and Anti-convulsant Effects of the Stem Bark Extract of Combretum hypopilinum in Laboratory Animals
Abubakar Kabiru , Usman Aminu, Yerima Musa , Muhammad Abubakaar Amali, Samaila Hassan, Abdullahi Suleiman, Rabiu Tijani Giaze and Abdulhamid Imam Tanko

Abstract:
Background and Objectives: Limited efficacy and negative property of currently available antiepileptic drugs limit their use and cause difficulties in patient management. The aim of this study was to evaluate the sedative and anti-convulsant effects of Combretum hypopilinum. Combretum hypopilinum stem bark was extracted using 95% methanol. Preliminary phytochemical screening of the crude methanol stem bark extract was carried out using standard procedures. Materials and Methods: Diazepam induced sleep was employed for the sedative effect. The anti-convulsant effect was evaluated by using MEST, PTZ and strychnine induced seizure models. Motor coordination deficit was also evaluated by using the beam walk assay method. Results: The result of the phytochemical screening revealed the presence of secondary metabolites such as; steroids, flavonoids and alkaloids. Acute toxicity studies were carried out and oral LD50 were found to be 3807.89 and >5000 mg kg1 b.wt., respectively. The extract showed a dose dependent and statistically significant decrease in the onset of sleep. The extract was active in maximal electroshock seizure test at doses of 200 and 400 mg kg1. The extract also exerted significant activity in Pentylenetetrazole (PTZ) seizure test at a dose of 400 mg kg1. The extract had no effect on Strychnine (STC) induced seizure test. In the beam walk assay test, the extract revealed no significant motor deficit in all tested doses. Conclusion: From the results of the present work, it may be concluded that the Combretum hypopilinum extract contains bioactive principles with central nervous system depressant effect, this may also support the traditional use of the plant in treatment of epilepsy and other mental illnesses.
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How to cite this article:

Abubakar Kabiru, Usman Aminu, Yerima Musa, Muhammad Abubakaar Amali, Samaila Hassan, Abdullahi Suleiman, Rabiu Tijani Giaze and Abdulhamid Imam Tanko, 2020. Sedative and Anti-convulsant Effects of the Stem Bark Extract of Combretum hypopilinum in Laboratory Animals. Journal of Pharmacology and Toxicology, 15: 58-64.

DOI: 10.3923/jpt.2020.58.64

URL: https://scialert.net/abstract/?doi=jpt.2020.58.64

 
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