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Journal of Pharmacology and Toxicology
  Year: 2020 | Volume: 15 | Issue: 1 | Page No.: 22-35
DOI: 10.3923/jpt.2020.22.35
 
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Intraperitoneal Acute Toxicity of Aluminum Hydroxide Nanoparticle as an Adjuvant Vaccine Candidate in Mice
Sjaikhurrizal El Muttaqien , Etik Mardliyati, Silmi Rahmani, Sabar Pambudi, Siti Maimunah, Ziah Izati Azkia, Nurmeilis , Asri Sulfianti, Julham Efendi, Damai Ria Setyawati, Asutiati Nurhasanah and Irvan Faizal

Abstract:
Background and Objective: Aluminum-containing adjuvant, such as aluminum hydroxide Al(OH)3), has been widely used and is generally regarded as safe to use in human vaccines. It is known that particle size of adjuvant plays critical role in dictating the adsorption of antigen. Numerous researches reported that down-sizing of Al(OH)3 into nano-sized particles could improve its immunopotentiation character. However, the safety of Al(OH)3 nanoparticle needs to be evaluated through acute toxicity study. This study aimed to assess biological character of nano-sized Al(OH)3, particularly its in vivo acute toxicity. Materials and Methods: Three types of samples including commercial Al(OH)3 of Alhydrogel®, Al(OH)3 microparticle (Mp) and Al(OH)3 nanoparticle (Np) were prepared and then peritoneally injected to BALB/c mice with 4 different doses (0.16, 1.28, 10.84 and 81.92 mg kg1 b.wt.). Post-administration, mice’s behavior and their body weight were recorded. After 14 days, mice were sacrificed and major organs were taken out for preparing hematoxylin-eosin (H and E) stains. Results: The average particle size of Alhydrogel®, Al(OH)3 Mp and Al(OH)3 Np were 4.02, 4.21 and 0.162 μm, respectively. The acute toxicity study showed that there is no lethal effect, clinical sign related complications and behavioral alteration even in highest administered dose for 14 days of observation period. In general, histological parameter of H and E stained tissue section among the treated groups was also comparable to those of control group, revealing relatively safe character of Al(OH)3 Np after peritoneal administration. Conclusion: Therefore, these results confirmed the potency and safety of Al(OH)3 Np as an adjuvant vaccine candidate.
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How to cite this article:

Sjaikhurrizal El Muttaqien, Etik Mardliyati, Silmi Rahmani, Sabar Pambudi, Siti Maimunah, Ziah Izati Azkia, Nurmeilis , Asri Sulfianti, Julham Efendi, Damai Ria Setyawati, Asutiati Nurhasanah and Irvan Faizal, 2020. Intraperitoneal Acute Toxicity of Aluminum Hydroxide Nanoparticle as an Adjuvant Vaccine Candidate in Mice. Journal of Pharmacology and Toxicology, 15: 22-35.

DOI: 10.3923/jpt.2020.22.35

URL: https://scialert.net/abstract/?doi=jpt.2020.22.35

 
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