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Journal of Pharmacology and Toxicology
  Year: 2017 | Volume: 12 | Issue: 3 | Page No.: 111-119
DOI: 10.3923/jpt.2017.111.119
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Ameliorating Effects of Magnolol in Reducing the Toxicity Induced by Heavy Metal Lead in the Human Brain Cells

Challa Suresh and Prasanna Kumar Dixit

Background and Objective: Lead (Pb) is being currently used in various forms and is getting exposed to living systems via different routes. It is found to affect different metabolic functions including antioxidant defense system. The treatment procedure for the lead exposure till now is not well defined and the objective of the study was to evaluate the effects of magnolol in ameliorating the toxicity induced by Pb. Materials and Methods: Human SH-SY5Y cells were exposed to different concentrations (0.01-10 μM) of Pb for 48 h and the cell viability was determined and the IC50 was observed at 5 μM. Various markers such s glutathions, caspase-3 and PGE2 were measured. The obtained data was analyzed using the one-way analysis of variance (ANOVA) and the statistical significance was assumed at p<0.05. Results: Findings suggest that there was no change in the cell viability when the cells were pretreated with MGL with a range of concentration from 0-140 μM for 48 h. The cells were treated with 80 μM of magnolol (MGL) to demonstrate its protective effects if any and against the 5 μM Pb pretreated cells for 48 h. The results indicated that, there was a significant (p<0.05) increase in the cell viability at least by 23.1% when compared with Pb alone treated group. Further, to evaluate the protective effects of MGL intracellular glutathione, caspase-3 and prostaglandin E2 levels were quantified. Conclusion: The results clearly showed the decrease in oxidative stress, inflammation percentages and reduce the impact of apoptosis. In over all, MGL was found to be an effective antioxidant, anti-apoptotic and anti-inflammatory agent.
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How to cite this article:

Challa Suresh and Prasanna Kumar Dixit, 2017. Ameliorating Effects of Magnolol in Reducing the Toxicity Induced by Heavy Metal Lead in the Human Brain Cells. Journal of Pharmacology and Toxicology, 12: 111-119.

DOI: 10.3923/jpt.2017.111.119






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