Effects of Selected Boesenbergia Species on the Proliferation of Several Cancer Cell Lines


	Journal of Pharmacology and Toxicology
	Year: 2011 \| Volume: 6 \| Issue: 3 \| Page No.: 272-282 DOI: 10.3923/jpt.2011.272.282

Effects of Selected Boesenbergia Species on the Proliferation of Several Cancer Cell Lines

Ling Jing Jing, Mohd Fadzelly Abu Bakar, Maryati Mohamed and Asmah Rahmat

Abstract: The aim of this research was to determine the potential of Boesenbergia species collected from Sabah rainforest as anticancer remedy. This research was done to Boesenbergia rotunda, B. pulchella, B. pulchella var attenuata and B. armeniaca crude extracts on the in vitro proliferation of hormone-dependent breast cancer (MCF-7), non-hormone dependent breast cancer (MDA-MB-231), ovarian cancer (CaOV₃), colon cancer (HT-29) and cervix cancer (Hela) cell lines. The effects of the four Boesenbergia species (methanol extracts of leaves, stem and rhizome part) on the proliferation of MCF-7, MDA-MB-231, CaOV₃, HT-29 and Hela cell lines were measured using (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay. The crude extracts with IC₅₀value less than 100 μg mL^-1 were subjected to cell cycle analysis by using flow cytometry for DNA content determination. B. rotunda as positive control showed significant inhibitions towards all the cancer cell lines tested with IC₅₀ranging from 51.0±0.01 to 71.0±1.41 μg mL^-1. The remaining Boesenbergia species only showed positive cytotoxicity activities against MCF-7 cell lines especially B. pulchella var attenuata with IC₅₀value 93.0±2.83 μg mL^-1 and B. armeniaca with 94.5±0.71 μg mL^-1. In cell cycle analysis, B. rotunda crude extract arrested cell at sub-G1 phase while B. pulchella var attenuata arrested cell at G₂/M phase. From this study, it can be concluded that only B. pulchella var attenuata and B. armeniaca were effective against cellular proliferations of MCF-7. However, both species were not as effective as B. rotunda.

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How to cite this article:

Ling Jing Jing, Mohd Fadzelly Abu Bakar, Maryati Mohamed and Asmah Rahmat, 2011. Effects of Selected Boesenbergia Species on the Proliferation of Several Cancer Cell Lines. Journal of Pharmacology and Toxicology, 6: 272-282.

DOI: 10.3923/jpt.2011.272.282

URL: https://scialert.net/abstract/?doi=jpt.2011.272.282

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