Honey is acceptable in the medical profession as an antibacterial agent for the treatment of some diseases and infections resulting from wounds and burns. Antibacterial and antifungal properties of honey are well documented against a number of Gram positive and Gram-negative bacteria and vary with origin and processing. In this study, we attempted to assess the Melghat honey as therapeutic agent and recorded high quality broad-spectrum antimicrobial properties. The bacterial pathogens, Staphylococcus epidermidis,Staphylococcus aureus, Klebsiella pneumoniae,Escherichia coli, Salmonella typhimurium, Salmonella typhi and Proteus vulgaris were highly sensitive to Melghat honey. Thus, the Melghat honey can be used as an alternative to antibiotics as well as a dressing for burns and other wounds.