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Journal of Pharmacology and Toxicology

Year: 2007 | Volume: 2 | Issue: 8 | Page No.: 698-708
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Authors

T.N. Padma Sree

S. Krishna Kumar

A. Senthilkumar

Gopala Krishna Aradhyam

Sathyanarayana N. Gummadi

Keywords

The bark extract of Terminalia arjuna is an age old Ayurvedic prescription for cardiac ailments and has been reported to be particularly beneficial in improving cardiac muscle function. However the specific biological activity of individual components of T. arjuna bark is not yet clearly understood. In this study, bark extract of Terminalia arjuna in six different solvents i.e., ethanol, acetone, water, ethyl acetate, chloroform and hexane were evaluated for free radical scavenging property, total phenols and reducing properties. Among all the extracts of T. arjuna, crude ethanolic bark extract had high phenolics, high reducing power and high free radical scavenging activity indicating that it is the best extract to isolate antioxidant compounds which could be used for further studies. Maximum phenol content (894.95 GAE/mg extract) and radical scavenging property (~88%) was observed for ethanol extract. In vitro studies on the effect of the bark extract of T. arjuna on endogenous antioxidant enzyme catalase showed inhibition of catalase activity. Among all the extracts used, ethanolic extract showed significantly higher levels of inhibition of catalase activity. Dose dependent studies showed a concentration dependent linear increase in inhibition of catalase activity and further assessment of the kinetic parameters showed a specific and rare competitive-non competitive` kind of inhibition. Although it has been shown in earlier studies on animal models that the bark extract increases the catalase expression levels, in this study we observed that the bark extract does not enhance the catalase activity in vitro. This suggests the possible role of compounds in ethanolic bark extract of T. arjuna in catalase gene expression.
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