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Journal of Pharmacology and Toxicology

Year: 2007 | Volume: 2 | Issue: 8 | Page No.: 681-697
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Authors

Amal M. Youssef Moustafa

Ahmed I. Khodair

Mahmoud A. Saleh

Keywords

Research Article

Phytochemical Investigation and Toxicological Studies of Lipid Constituents Isolated from Leptadenia pyrotechnica

Amal M. Youssef Moustafa, Ahmed I. Khodair and Mahmoud A. Saleh
Investigation of the chemical constituents of Leptadenia pyrotechnica (Asclepiadaceace family) let to isolate three terpenes; phytol, squalene and taraxerol, five sterols; cholesterol, campasterol, stigmasterol, β-sitosterol and fucosterol. Fifteen fatty acids were isolated (C14-C25), eleven n-alkanol (C29-C39), series of n-alkanes (C12-C36), one n-alkene; 3-tetradecene. Also, for the first time eighteen aromatic hydrocarbons were isolated. 5-Phenyl-Undecane and 6-Phenyl-Tridecane are the major constituents. The structures of these compounds were established by Gas chromatography; GC-FID and GC-MS, spectroscopic techniques; Infra-red (IR) and comparison with the published data. The unsaponifiable matter was divided into two parts: The first part was directly subjected to GC/FID and GC/MS chromatographic analysis. While the second part, was subjected to column chromatographic fractionation. The isolated fractions were identified as fatty alcohols, hydrocarbons, terpenes and sterols. Adduction method was used to separate the straight-chain fatty acids.The acute toxicity study of the total lipid extract was examined on brine shrimp, the LC50 was 35.48 ppm. The extract is highly toxic.
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