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Journal of Pharmacology and Toxicology
  Year: 2007 | Volume: 2 | Issue: 5 | Page No.: 437-446
DOI: 10.3923/jpt.2007.437.446
Novel Use of Uric Acid and Sodium Arsenite to Induce Vascular Endothelial Dysfunction in Rats
Pitchai Balakumar, Seema Jindal and Manjeet Singh

Abstract:
The present study has been designed to investigate the potential of in-vivo administration of uric acid and sodium arsenite in the development of vascular endothelial dysfunction (VED) in rats. The uric acid (100, 150, 200 mg kg-1 day-1, i.p., 3 weeks) and sodium arsenite (1, 1.5, 2 mg kg-1 day-1, i.p., 2 weeks) were administered to rats. Vascular endothelial dysfunction was assessed by employing isolated aortic ring preparation, electron microscopy of thoracic aorta and estimating serum concentration of nitrite/nitrate. Further, serum thiobarbituric acid reactive substances (TBARS) and aortic production of superoxide anion were estimated to assess oxidative stress. High dose of uric acid (200 mg kg-1 day-1, i.p., 3 weeks) and sodium arsenite (2 mg kg-1 day-1, i.p., 2 weeks) were noted to produce high mortality rate (>85%) in animals. On the other hand, less mortality rate (<5%) was observed in animals treated with uric acid (100, 150 mg kg-1 day-1, i.p., 3 weeks) and sodium arsenite (1, 1.5 mg kg-1 day-1, i.p., 2 weeks). Moreover, uric acid (100, 150 mg kg-1 day-1, i.p., 3 weeks) and sodium arsenite (1, 1.5 mg kg-1 day-1, i.p., 2 weeks) were noted to produce vascular endothelial dysfunction by attenuating acetylcholine-induced endothelium dependent relaxation, impairing the integrity of vascular endothelial lining, decreasing serum nitrite/nitrate concentration and increasing serum TBARS and aortic superoxide anion generation. Hence, it may be concluded that uric acid (100 to 150 mg kg-1 day-1, i.p., 3 weeks) and sodium arsenite (1 to 1.5 mg kg-1 day-1, i.p., 2 weeks) may be employed as potential chemically-induced models to produce vascular endothelial dysfunction in rats.
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How to cite this article:

Pitchai Balakumar, Seema Jindal and Manjeet Singh , 2007. Novel Use of Uric Acid and Sodium Arsenite to Induce Vascular Endothelial Dysfunction in Rats. Journal of Pharmacology and Toxicology, 2: 437-446.

DOI: 10.3923/jpt.2007.437.446

URL: https://scialert.net/abstract/?doi=jpt.2007.437.446

 
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