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Journal of Plant Sciences
  Year: 2020 | Volume: 15 | Issue: 2 | Page No.: 54-63
DOI: 10.3923/jps.2020.54.63
 
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Callus Induction and Establishment of Cell Suspension Culture of Cumin (Cuminum cyminum L.)
S. Suthar Ramchandra , P.N. Bhatt and D.P. Bhatt

Abstract: Background and Objective: Resistance to diseases is an important step for the establishment of a genetic transformation system in plants. In current research, callus induction and establishment of cell suspension culture were investigated in Cuminum cyminum. Materials and Methods: Callus inducted from hypocotyls segments. The experiment was arranged in a factorial experiment with 2,4-Dichlorophenoxyacetic acid (2,4-D) and Kinetin (KIN) different concentrations (0, 0.1, 1 and 10 μM) on MS medium. Callus was inducted 95.6% on MS medium supplements with 2, 4-D 0.1 and KIN 10 μM L–1. The cell suspension culture was established from the callus of Cumin GC-4. Using different concentrations and combinations of 2,4-D and kinetin the growth patterns of cell suspension cultures were examined during a range of culture periods (0, 7, 14, 21 and 28 days). Results: The growth rates of cells were initially slow, but as the culture proceeded, they increased significantly and accumulated great amounts of biomass over a period of 28 days. Conclusion: Friable callus production is important in establishment of good quality of cell suspension. Researcher can take benefit from its investigation to manipulation genetically.
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How to cite this article:

S. Suthar Ramchandra, P.N. Bhatt and D.P. Bhatt, 2020. Callus Induction and Establishment of Cell Suspension Culture of Cumin (Cuminum cyminum L.). Journal of Plant Sciences, 15: 54-63.

DOI: 10.3923/jps.2020.54.63

URL: https://scialert.net/abstract/?doi=jps.2020.54.63
 
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