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Journal of Plant Sciences
  Year: 2015 | Volume: 10 | Issue: 1 | Page No.: 15-24
DOI: 10.3923/jps.2015.15.24
Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds
Chirom Oceania, Thingujam Doni, Leimapokpam Tikendra and Potshangbam Nongdam

Abstract:
Tomato is a highly popular crop for its rich nutritional values and constitutes an integral part of daily diets for substantial portion of human population of the world. Because of limitations in conventional breeding methods and increasing demand of this important crop for fast growing population, large scale propagation of tomato through plant tissue culture techniques becomes highly significant. Efficient in vitro generation protocols for tomato have been established successfully by employing seed and tissue culture derived shoot-tip explants. 6-benzyl amino purine (BAP) was highly effective in inducing seed germination and rapid leaf multiplication though presence of 0.4% activated charcocal (AC) had negative influence on culture development. The used of in vitro derived shoot-tips as explants removed the deleterious effect of AC resulting in the production of highest number of leaves in MS+3 mg L-1 BAP+0.5 mg L-1 NAA+AC. The Murashige and Skoog (MS) medium augmented with as 1-naphthalene acetic acid (NAA) was not favourable for seed germination but germination rate and root multiplication responses accelerated with the incorporation of AC in medium. Maximum number of roots differentiated from the germinating seeds in MS+3 mg L-1 NAA+1 mg L-1 BAP+AC. The MS medium supplemented with 1.5 mg L-1 NAA generated highest root formation when shoot-tip propagules were used for culture initiation. Preliminary investigation of synthetic seed development of tomato revealed the gel matrix produced from the complexation between 2% sodium alginate and 100 mM CaCl2 for 20-40 min as the most favourable for efficient encapsulation of in vitro derived shoot-tip propagules. The in vitro protocols developed from the present investigation can be utilized for propagation of tomato in larger scale for commercial purposes as well as local domestic consumption.
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How to cite this article:

Chirom Oceania, Thingujam Doni, Leimapokpam Tikendra and Potshangbam Nongdam, 2015. Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds. Journal of Plant Sciences, 10: 15-24.

DOI: 10.3923/jps.2015.15.24

URL: https://scialert.net/abstract/?doi=jps.2015.15.24

 
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