Submit Manuscript

Journal of Plant Sciences

Year: 2011 | Volume: 6 | Issue: 4 | Page No.: 182-189
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 358

Search

Authors

Muneera D.F. Alkahtani

M.M. Mazen

M.A. El- Naggar

M.K. Arfa

Keywords

Research Article

The Relationship Between Some Mycotoxins Excretion and Bean Seed Discoloration

Muneera D.F. Alkahtani, M.M. Mazen, M.A. El- Naggar and M.K. Arfa
Mycotoxins is a serious problem threatening human and animal health and detection is the most important steps to get rid of them. The impact of these mycotoxins on seed physiological characteristics as discoloration is important things that are related with seed quality as well as marketing. The fungal isolation was conducted from three cultivars of bean seeds (Giza3, Giza4 and Giza6) after dividing into groups depending on their color. Sixteen fungal species were associated with discolored bean seed samples. The Aflatoxin (AF), Alternariol (AOH) and Zearalenone (ZON) were determined using immunoaffinity columns and GC-MS. Aflatoxin was existed in all colored seeds while, the dark brown colored seeds contaminated with AF, AOH and ZON. The highest concentration was found in dark brown colored seeds followed by golden brown. However, Zearalenone (ZON) was detected only in the dark brown in high concentration (45 μg kg-1 seeds). AOH toxin excreted by both isolates of A. alternata ranged from 1.0 to 3.0 μg g-1. The highest level of AOH toxin has been detected after 14 days of incubation and then decreased. Whereas, the normal seeds contained the lowest concentration of Aflatoxin. The amount of Aflatoxin produced by A. parasiticus increased positively with increasing incubation time. The amount reached 200 (μg kg-1) after 35 days incubation. Nevertheless, this amount was decreased to 125 (μg kg-1) after some period when bean seeds simultaneously inoculated with A. alternata and A. parasiticus. In conclusion mycotoxins affect on the seed physiology, there is a direct relationship between the rate of mycotoxins (AF, AOH and ZON) contamination and the degree of discoloration. The presence of more than fungus together may result in lack of mycotoxins content.
PDF Fulltext XML References Citation

Leave a Comment

Your email address will not be published. Required fields are marked *