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Journal of Plant Sciences
  Year: 2008 | Volume: 3 | Issue: 4 | Page No.: 236-247
DOI: 10.3923/jps.2008.236.247
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Physiological and Molecular Characterization of Rice Isogenic Line for SubQTL9 under Flash Flooding

V. Ruanjaichon, T. Toojinda, S. Tragoonrung and A. Vanavichit

Discovering QTL associated with submergence tolerance facilitates the development of isogenic lines for submergence stress. One hundred and thirty two isogenic lines carry 36 kb region contained in the QTLchr9 were used to compare under controlled submerging conditions. The region contained gene encoding three ethylene responsive element binding protein as Sub1A, OsEREBP1 (Sub1C), OsEREBP2 (Sub1B) genes. The Isogenic Lines (ISLs) were categorized into three groups as homozygous donors (DH206), ISLs-CC, homozygous recipients (KDML105), ISLs-cc and heterozygous, ISLs-Cc, by the three pairs of primers targeted at the target region. Each individual plant of the ISLs was submerged for 20 days to increase the physiological changes in response to flooding. In addition the increment in shoot height, plant growth and the ability to stay green of ISLs-CC and ISLs-Cc were also similar. Rapid elongation was significantly higher in ISLs-cc. All genotypes showed no significance with respect to leaf senescence. However they were shown to be significant when genotypes interacted to time-course. The accumulation of shoot elongation was affected mostly by the extension of leaf blade and leaf sheath. A prolonged flooding to reach a tolerance plant was associated with the plant adaptation of suppressing growth and the maintenance of green leaf area. However several lines within each class did not differ in plant adaptations under normal conditions. The analysis of variance for the genotypic effect (G) and time (T) duration showed that the genotype main effects (G) from CC, Cc and cc were highly significant at 1% level, meaning that differences between genotypes vary across time. Plant Elongation (PE), Total Shoot Elongation (TSE) and Relative Shoot Elongation (RSE) showed high negative significance (p<0.05) correlated with percent plant survival as r = -0.78*, -0.75* and -0.34*. In contrast, percent plant survival showed a high positive correlation (r = 0.71*)with Leaf Senescence (LS). There were significant differences in plant elongation and leaf senescence among classes. This study provides a powerful tool to improve flooding tolerance in plants.
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How to cite this article:

V. Ruanjaichon, T. Toojinda, S. Tragoonrung and A. Vanavichit, 2008. Physiological and Molecular Characterization of Rice Isogenic Line for SubQTL9 under Flash Flooding. Journal of Plant Sciences, 3: 236-247.

DOI: 10.3923/jps.2008.236.247






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