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Journal of Plant Sciences
  Year: 2007 | Volume: 2 | Issue: 1 | Page No.: 35-44
DOI: 10.3923/jps.2007.35.44
 
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Somatic Embryogenesis of Musa sp. cv. Kanthali Using Floral Bud Explants

Sarder Nasir Uddin and Soubir Titov

Abstract:
The study was undertaken to examine banana (Musa sp. cv. Kanthali) floral bud apex as an alternative source material for in vitro propagation because huge number of explants die due to microbial contamination in case of shoot tip explants. Contamination free cultures were established by treating the floral bud explants with 0.1% HgCl2 for 6 min. For reducing phenolic compounds secretion, explants were treated with 0.125% potassium citrate: citrate solution. Compact, white/greenish white callus was formed in different amount at all concentrations of BA+Kn+IAA+15% CW and BA+Kn+IBA+15% CW from terminal floral bud explants after 3 weeks of culture. All were again subcultured at same medium and after another 30-35 days at 2.0 mg L-1 BA+2.0 mg L-1 Kn+2.0 mg L-1 IAA+15% CW some callus showed embryogenic structure.
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How to cite this article:

Sarder Nasir Uddin and Soubir Titov, 2007. Somatic Embryogenesis of Musa sp. cv. Kanthali Using Floral Bud Explants. Journal of Plant Sciences, 2: 35-44.

DOI: 10.3923/jps.2007.35.44

URL: https://scialert.net/abstract/?doi=jps.2007.35.44

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