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Journal of Plant Sciences
  Year: 2007 | Volume: 2 | Issue: 3 | Page No.: 347-352
DOI: 10.3923/jps.2007.347.352
 
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Pectin Lyase Activity in Culture Filtrate of Penicillium Species

A.O. Adejuwon and P.O. Olutiola

Abstract:
Penicillium species grew in a synthetic medium with pectin as the sole carbon source producing proteins which expressed alkaline pectin lyase activity. The enzyme was purified by a combination of ammonium sulphate precipitation, dialysis, gel filtration and ion-exchange chromatography. Optimum activity of the enzyme was at 35°C and at pH 80. The enzyme was stimulated by K+, Na+, Ca++ and Mg++ but inhibited by EDTA and Hg++. The enzyme was able to degrade pectin with optimum activity expressed at 16 mg mL‾1.
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How to cite this article:

A.O. Adejuwon and P.O. Olutiola , 2007. Pectin Lyase Activity in Culture Filtrate of Penicillium Species. Journal of Plant Sciences, 2: 347-352.

DOI: 10.3923/jps.2007.347.352

URL: https://scialert.net/abstract/?doi=jps.2007.347.352

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