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Journal of Plant Sciences
  Year: 2007 | Volume: 2 | Issue: 3 | Page No.: 334-340
DOI: 10.3923/jps.2007.334.340
 
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Pectin Lyase Activity in Culture Medium of Lasidioplodia theobromae

A.O. Adejuwon and P.O. Olutiola

Abstract:
Lasidioplodia theobromae grew in a culture medium at 27 °C producing proteins which showed pectin lyase activity. The enzyme was purified by a combination of ammonium sulphate precipitation, dialysis, gel permeation and ion-exchange chromatography. Optimum activity of the enzyme was at 40°C and at pH 8.5. The enzyme was stimulated by concentrations of K+, Na+, Ca++ and Mg++ but inhibited by EDTA and Hg++. When the enzyme was heated at 70 °C, optimum activity had decreased by about 66.6% within 2 min but almost completely lost within 30 min. The enzyme was able to degrade pectin with optimum activity expressed at 18-20 mg mL-1.
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How to cite this article:

A.O. Adejuwon and P.O. Olutiola , 2007. Pectin Lyase Activity in Culture Medium of Lasidioplodia theobromae. Journal of Plant Sciences, 2: 334-340.

DOI: 10.3923/jps.2007.334.340

URL: https://scialert.net/abstract/?doi=jps.2007.334.340

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