
	Journal of Plant Sciences
	Year: 2007 \| Volume: 2 \| Issue: 3 \| Page No.: 273-282 DOI: 10.3923/jps.2007.273.282

Determination of Phenolic Acetophenones in Radix Cynanchi Paniculati by Capillary Electrophoresis with Electrochemical Detection

Xiao Yao, Jianxia Zhang and Gang Chen

Abstract: Capillary electrophoresis with electrochemical detection has been employed for the determination of three phenolic acetophenones (including paeonol, p-hydroxyacetophenone and 2,4-hydroxyacetophenone) in Radix Cynanchi Paniculati (i.e., Paniculate Swallowworf Root) for the first time. Effects of several important factors such as the concentration and the acidity of the running buffer, separation voltage, injection time and detection potential were investigated to acquire the optimum conditions. The detection electrode was a 300 μm diameter carbon disc electrode at a working potential of +0.90 V (vs. Saturated Calomel Electrode (SCE)). The three analytes can be well separated within 10 min in a 50 cm length fused silica capillary at a separation voltage of 15 kV in a 50 mM borate buffer (pH 9.2). The relation between peak current and analyte concentration was linear over about 3 orders of magnitude with the detection limits (S/N = 3) ranging from 0.20 to 0.36 μM for all analytes. The proposed method has been successfully applied to the determination of the bioactive constituents in the real herbal samples with satisfactory assay results.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	The Synthesis of Benzopyran Analogues with Variation at C-2, C-4 and C-7 Positions

How to cite this article:

Xiao Yao, Jianxia Zhang and Gang Chen , 2007. Determination of Phenolic Acetophenones in Radix Cynanchi Paniculati by Capillary Electrophoresis with Electrochemical Detection. Journal of Plant Sciences, 2: 273-282.

DOI: 10.3923/jps.2007.273.282

URL: https://scialert.net/abstract/?doi=jps.2007.273.282

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		FSTA
		Google Scholar
		ProQuest

Science Alert