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Journal of Plant Sciences
  Year: 2006 | Volume: 1 | Issue: 1 | Page No.: 42-48
DOI: 10.3923/jps.2006.42.48
Plant Regeneration from Axillary Shoot Segments Derived Callus in Hemidesmus indicus (L.) R. Br. (Anantamul) an Endangered Medicinal Plant in Bangladesh
N.A. Siddique and M.A. Bari

Abstract:
Callus cultures were obtained from axillary shoots of Hemidesmus indicus on (MS) medium supplemented with 2,4-D, NAA, IAA and Kn. The highest frequency (85.00%) of organogenic callus induction was observed in MS medium containing 1.0 mg L-1 NAA and 2.0 mg L-1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP, NAA, IAA and Kn. Shoots differentiated best (90.00%) from axillary shoot derived callus on MS medium containing 1.0 mg L-1 NAA and 2.5 mg L-1 Kn. Regenerated shoots rooted best in MS supplemented in the IBA and Kn. Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
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How to cite this article:

N.A. Siddique and M.A. Bari , 2006. Plant Regeneration from Axillary Shoot Segments Derived Callus in Hemidesmus indicus (L.) R. Br. (Anantamul) an Endangered Medicinal Plant in Bangladesh. Journal of Plant Sciences, 1: 42-48.

DOI: 10.3923/jps.2006.42.48

URL: https://scialert.net/abstract/?doi=jps.2006.42.48

 
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