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Journal of Plant Sciences
  Year: 2006 | Volume: 1 | Issue: 3 | Page No.: 192-200
DOI: 10.3923/jps.2006.192.200
 
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RAPD Based Fingerprinting of Tomato Genotypes for Identification of Mutant and Wild Cherry Specific Markers

Manoj Kulkarni and Uday Deshpande

Abstract:
Ten tomato (Lycopersicon esculentum) genotypes showing distinct variation in morphological and anatomical features were screened for random amplified polymorphic DNA (RAPD). Germplasm under study comprised 3 mutant derivatives, 3 hybrids, their parents and one wild cherry tomato genotype. Twelve random decamer operon primers of OPAB series could generate total 690 bands of which 33.3% were polymorphic. Mutant specific polymorphic markers were detected. Polymorphy could clearly identify mutant derivatives, cultivated genotypes and wild cherry tomato genotype. The UPGMA based dendrogram divided genotypes into two main clusters. Interestingly, wild cherry tomato was present in cluster of mutant derivatives, affirming its potential for utilization as unique source for tomato breeding. Low level of genetic diversity was noted in cultivated tomato genotypes (Mean = 0.138) indicating the existence of limited or narrow genetic base.
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How to cite this article:

Manoj Kulkarni and Uday Deshpande , 2006. RAPD Based Fingerprinting of Tomato Genotypes for Identification of Mutant and Wild Cherry Specific Markers. Journal of Plant Sciences, 1: 192-200.

DOI: 10.3923/jps.2006.192.200

URL: https://scialert.net/abstract/?doi=jps.2006.192.200

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