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Research Journal of Parasitology
  Year: 2018 | Volume: 13 | Issue: 2 | Page No.: 47-56
DOI: 10.3923/jp.2018.47.56
 
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Comparative Serodiagnostic Evaluation of Tegumental Antigen and its 16.5 KDa Subunit to Crude Antigens in Human Fascioliasis
I.M. Abdelsalam, R.M. Sarhan , A.F. Badawy and G.H. Shinkar

Abstract:
Background and Objectives: Enzyme-linked Immunosorbent Assay (ELISA) was considered the most sensitive and specific adjuvant to fecal analysis for diagnosis of fascioliasis. Since specificity of ELISA depended mainly on the used antigens and history of tested sera, so the present study was designed to comparatively evaluate the diagnostic performance of Tegumental (T) Antigen (Ag) and its 16.5 KDa subunit to Somatic (S) and Excretory-Secretory (E-S) Ags of F. gigantica in an indirect total IgG-ELISA for serological diagnosis of human infection. Materials and Methods: The 16.5 KDa T Ag subunit was prepared by SDS polyacrylamide gel electrophoresis from T Ag followed by purification and elution of the selected band by Pall Nanosep microcentrifugal device also the S, E-S and T Ags were prepared. Their immunodiagnostic performance was compared in an indirect total IgG ELISA. Results: The ROC analysis revealed the highest sensitivity from the T Ag (100%) followed by SAg (86.6%), E-S Ag (73.3%) and the least value was shown from the 16.5 KDa subunit (53.3%). Again, as regard specificity, the highest value was obtained from T Ag (100%) followed by E-S Ag (79.2%) then the 16.5 KDa subunit (75%) and least value were obtained from SAg (70.8%). Conclusion: The F. gigantica T Ag was superior to S, E-S and the 16.5 KDa subunit in the serological diagnosis. This performance was followed by S and E-S Ags. Unexpectedly, the purified 16.5 KDa T subunit showed a poor diagnostic outcome.
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How to cite this article:

I.M. Abdelsalam, R.M. Sarhan, A.F. Badawy and G.H. Shinkar, 2018. Comparative Serodiagnostic Evaluation of Tegumental Antigen and its 16.5 KDa Subunit to Crude Antigens in Human Fascioliasis. Research Journal of Parasitology, 13: 47-56.

DOI: 10.3923/jp.2018.47.56

URL: https://scialert.net/abstract/?doi=jp.2018.47.56

 
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