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Research Journal of Parasitology
  Year: 2018 | Volume: 13 | Issue: 2 | Page No.: 36-46
DOI: 10.3923/jp.2018.36.46
 
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Mini-FLOTAC Versus Other Copromicroscopic Methods in Diagnosis of Intestinal Parasitic Infections
Rania Mohammad Sarhan , Yasmeen Mohammad Shaaban, Aisha Tawfik Hassan and Hala Said Salem

Abstract:
Background and Objective: Mini-FLOTAC proved to be a good promising quantitative method combining both sensitivity and low costs. This study aimed to assess the real efficiency of Mini-FLOTAC vs. other copro-parasitological methods, wet smear, sedimentation and flotation concentration for diagnosis of intestinal parasitic infections. Materials and Methods: Three studies were carried out. The first was used to evaluate the performance and sensitivity. The second was designed to evaluate the percent accuracy, precision and sensitivity and the third, a field study, for validating its sensitivity and predictive value. Results: When applying different flotation solutions (FS). Mini-FLOTAC revealed the highest sensitivity from FS1 and FS3 for detection of H. nana and E. vermicularis, FS1 for A. lumbricoides and FS3 for E. histolytica. Samples with lower level of enrichment had a higher coefficient of variation and a lower precision. The second study was designed to evaluate its accuracy (%), precision and sensitivity. The highest sensitivity was obtained for H. nana eggs, this value was slightly lower for A. lumbricoides eggs and the lowest value was obtained for E. histolytica cysts. A high linear relationship was revealed between outcomes. The third study, a field one for validating the sensitivity and predictive value. Out of 200 children, 38 positive cases were detected. Results revealed that FS3 showed the highest outcome. The performance of all methods on negative individuals was high (NPV>95%) for all parasites. The NPV for detection of H. nana eggs and E. vermicularis eggs with Mini-FLOTAC was 100% by the two FSs while it decreased in case of G. intestinalis to be 97.8 and 96.2% by FS1 and FS3, respectively. The KI for agreement among techniques showed a nearly perfect comparative relation. Conclusion: Mini-FLOTAC proved obvious sensitivity in diagnosis of helminths but more studies are needed to assess its capability in protozoa detection.
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How to cite this article:

Rania Mohammad Sarhan, Yasmeen Mohammad Shaaban, Aisha Tawfik Hassan and Hala Said Salem, 2018. Mini-FLOTAC Versus Other Copromicroscopic Methods in Diagnosis of Intestinal Parasitic Infections. Research Journal of Parasitology, 13: 36-46.

DOI: 10.3923/jp.2018.36.46

URL: https://scialert.net/abstract/?doi=jp.2018.36.46

 
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