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Research Journal of Parasitology

Year: 2017 | Volume: 12 | Issue: 2 | Page No.: 33-44

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Authors


Shaimaa Helmy  El-Sayed

Shaimaa Helmy El-Sayed

LiveDNA: 20.16082

Neimat Amer

Country: Egypt

Soad Ismail

Country: Egypt

Iman Ali

Country: Egypt

Enas  Rizk

Enas Rizk

LiveDNA: 20.16589

Mona  Magdy

Mona Magdy

LiveDNA: 20.16581

Ayman Abdel-Moamen  El-Badry

Ayman Abdel-Moamen El-Badry

LiveDNA: 20.277

Keywords


  • ultrastructure
  • Blastocystis
  • bee pollen compound
  • olive leaf extract
  • metronidazole
  • genotyping
Research Article

In Vitro and In Vivo Anti-blastocystis Efficacy of Olive Leaf Extract and Bee Pollen Compound

Shaimaa Helmy El-Sayed Shaimaa Helmy  El-Sayed's LiveDNA, Neimat Amer, Soad Ismail, Iman Ali, Enas Rizk Enas  Rizk's LiveDNA, Mona Magdy Mona  Magdy's LiveDNA and Ayman Abdel-Moamen El-Badry Ayman Abdel-Moamen  El-Badry's LiveDNA
Background: Blastocystis is the most common enteric protozoa in humans and animals worldwide. The hazardous side effects and potential development of drug resistance to the standard drug, metronidazole (MTZ), necessitate the search for safer and more efficient alternative therapeutics. Objective: The present study was designed to investigate the in vitro activity and in vivo efficacy of two natural medicinal compounds, Olive Leaf Extract (OLE) and Bee Pollen Compound (BPC) against subtype 3 Blastocystis isolated from symptomatic patients. Materials and Methods: Cultured parasites were challenged with a graded concentration of OLE (500, 1000 μg mL–1), BPC (500, 1000 μg mL–1) and MTZ (150, 250 μg mL–1) to assess its in vitro growth by counting the number of viable cells after one and two h of incubation and morphological ultrastructural changes were evaluated using Transmission Electron Microscope (TEM). The anti-Blastocystis effects of OLE and BPC were determined in 40 laboratory bred Swiss albino mice inoculated with Blastocystis isolates using parasitological, histopathological and immunohistochemical methods. Results: Blastocystis isolated from symptomatic patients were subtype 3. The OLE and BPC showed statistically significant (p<0.05) in vitro growth inhibition of Blastocystis in a concentration dependent manner and a statistically significant (p<0.05) in vivo reduction of the number of Blastocystis in stool samples and intestinal contents of infected treated mice higher than MTN effects with apoptotic-like death and Programmed Cell Death (PCD) in Blastocystis. The BPC normalized villous architecture and increased local IgA secretory villous cells. Conclusion: These findings highlighted the potential therapeutic effects of OLE and BPC as potent safe natural alternatives against blastocystosis. The BPC was superior to OLE in ameliorating the severity of tissue pathology and had an immunostimulatory effect on intestinal cells.
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How to cite this article

Shaimaa Helmy El-Sayed, Neimat Amer, Soad Ismail, Iman Ali, Enas Rizk, Mona Magdy and Ayman Abdel-Moamen El-Badry, 2017. In Vitro and In Vivo Anti-blastocystis Efficacy of Olive Leaf Extract and Bee Pollen Compound. Research Journal of Parasitology, 12: 33-44.

URL: https://scialert.net/abstract/?doi=jp.2017.33.44

Comments


Amira Mahmoud Eisa Reply
15 October, 2020

very helpful paper, kindly I want to contact the author

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