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Research Journal of Parasitology
  Year: 2011 | Volume: 6 | Issue: 1 | Page No.: 53-59
DOI: 10.3923/jp.2011.53.59
 
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Inhibition of Trypanosoma brucei brucei by Extracts from Waltheria indica L. (Sleepy Morning)
A.Y. Bala, T. Adamu, U. Abubakar and M.J. Ladan

Abstract:
The study was conducted to evaluate the inhibitory activity of polar and non-polar extracts from Waltheria indica against Trypanosoma brucei brucei, the causative parasite of trypanosomosis, for the first time. The parasites Trypanosoma brucei brucei were cultured using 96 well micro titer plate and maintained at 37°C. About 20-25 parasites per microscope field were dozed with 1, 2 and 4 mg mL-1 of the extracts of the plant. Two controls were set up; one is Berenil (the reference drug), which was used as positive control and a negative control group without extract/Berenil. Results showed that the ethanol extract was the most active with Inhibitory Concentration (IC50) value of 0.5-1 mg mL-1 while the petroleum ether extract was moderate against the trypanosome parasites with IC50 value of 3.8-4.0 mg mL-1. The results also showed that the parasites survived more than 4 h when no plant extract/berenil was present. Phytochemical screening revealed a range of compounds including, alkaloids, terpenes, flavonoids, tannins, glycosides, steroids, among others, which could be responsible for the trypanocidal activity of the extracts. Further studies including in vivo assays for the determination of efficacy and toxicity are planned and will be carried out.
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How to cite this article:

A.Y. Bala, T. Adamu, U. Abubakar and M.J. Ladan, 2011. Inhibition of Trypanosoma brucei brucei by Extracts from Waltheria indica L. (Sleepy Morning). Research Journal of Parasitology, 6: 53-59.

DOI: 10.3923/jp.2011.53.59

URL: https://scialert.net/abstract/?doi=jp.2011.53.59

 
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