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Research Journal of Parasitology

Year: 2010 | Volume: 5 | Issue: 3 | Page No.: 139-147
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Authors

A.C. Franca-Botelho

J.L. Franca

E.L. Franca

A.C. Honorio-Franca

H.G.N. O. Busatti

M.A. Gomes

Keywords

This study aimed to evaluate the phagocytic ability of Entamoeba histolytica and Entamoeba dispar strains and the oxidative stress response. Erythrocytes and mononuclear (MN) and polymorphonuclear (PMN) leukocytes, were separated from peripheral blood. The samples were fractionated over a Ficoll-Paque density gradient. Then they were incubated with trophozoites of the virulent E. histolytica (HM1-IMSS) and avirulent (ICB-32) strain and E. dispar (ICB-ADO) nonpathogenic. Superoxide anion was determined by its reaction with cytochrome chromogen and SOD release by the nitroblue tetrazolium reduction test. The results show that the virulence of E. histolytica was correlated to their phagocytic activity. The virulent strain indeed presented greater erythrophagocytic and leuchophagocytic activity. The interaction between MN and E. histolytica provoked larger SOD release and smaller superoxide concentration compared to the values obtained in the PMN and E. histolytica interaction, where the largest concentration of superoxide was observed in the PMN and ICB-32 interaction, which caused significant amoeba death. The results indicate that these E. histolytica virulent strains are more efficient in inhibiting the oxidative burst of MN and that avirulent strains stimulate the production of superoxide for PMN, making the latter more susceptible to death.
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How to cite this article

A.C. Franca-Botelho, J.L. Franca, E.L. Franca, A.C. Honorio-Franca, H.G.N. O. Busatti and M.A. Gomes, 2010. Relationship Between Oxidative Stress Production and Virulence Capacity of Entamoeba Strains. Research Journal of Parasitology, 5: 139-147.

URL: https://scialert.net/abstract/?doi=jp.2010.139.147

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