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Journal of Medical Sciences
  Year: 2018 | Volume: 18 | Issue: 4 | Page No.: 205-210
DOI: 10.3923/jms.2018.205.210
 
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Effect of Channa micropeltes for Increasing Lymphocyte and Fibroblast Cells in Diabetic Wound Healing

Maharani Laillyza Apriasari and Dewi Puspitasari

Abstract:
Background and Objective: Diabetes mellitus (DM) has the complications in the form of a wound healing process which take a long time. The DM patients of Indonesia have ranks 4th largest in the world. Empirically the people of South Kalimantan believe that Haruan fish (Channa striata) and Toman fish (Channa micropeltes) can accelerate wound healing. Both of them have the same genus that contain omega-6, albumin and zinc which can accelerate wound healing. To analyze the effect of toman fish extract orally at a dose of 16 mL kg1 of rat as alternative therapy of diabetic wound healing by increasing the number of lymphocyte and fibroblast cells on the 2nd, 4th and 8th days. Materials and Methods: This study was experimental with post test only control design. The sample used 36 male wistar rats, 200-250 mg weight as the model of diabetic wound healing on the back of skin. The study consisted of 3 groups: negative control (only fed), treatment (given toman fish extract 16 mL kg1) and positive control (given haruan fish extract 13.54 mL kg1) given for 2 times a day. On day 2, 4 and 8 rats were sacrificed and did the biopsy, then did the histopathology examination by Hematoxylin Eosin to see the number of lymphocytes and fibroblast cells. Results: Shapiro Wilk normality test (p>0.05) and Variance Levene's Homogeneity Test (p>0.05) obtained normal data distribution results and homogeneous data variance, then followed by LSD post hoc test. The highest mean value of the number of lymphocytes in the treatment group for the 4th days (15.20±0.84) and the lowest in the negative control group for the 2nd day (5.60±2.30). The highest mean value of the number of fibroblasts in the 4th day treatment group (24.20±1.79) and the lowest in the negative control group 2nd day (10.60±1.14). Conclusion: The administration of toman fish extract orally at a dose of 16 mL kg1 BB of rat can be alternative therapy of diabetic wound healing by increasing the highest lymphocyte and fibroblast cells on 4th day.
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How to cite this article:

Maharani Laillyza Apriasari and Dewi Puspitasari, 2018. Effect of Channa micropeltes for Increasing Lymphocyte and Fibroblast Cells in Diabetic Wound Healing. Journal of Medical Sciences, 18: 205-210.

DOI: 10.3923/jms.2018.205.210

URL: https://scialert.net/abstract/?doi=jms.2018.205.210

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