Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Journal of Medical Sciences
  Year: 2016 | Volume: 16 | Issue: 1-2 | Page No.: 16-24
DOI: 10.3923/jms.2016.16.24
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Effect of Bone Marrow-derived Mesenchymal Stem Cells on Changes of Serum Levels of TNF-α and Locomotor Function after Spinal Cord Injury in Mice

Noushin Gashmardi, Davood Mehrabani , Zahra Khodabandeh and Seyed Mojtaba Hosseini

Abstract:
Spinal Cord Injury (SCI) is still a devastating clinical problem with irreversible consequences leading to permanent functional loss and life time disability. This study was conducted to assess the healing effect of bone marrow-derived mesenchymal stem cells on locomotor function and changes of Tumor Necrosis Factor alpha (TNF-α) after SCI in mice. Forty two BALB/C mice were divided into 3 equal groups of control, SCI and treatment [transplantation of 5×104 Bone Marrow Stem Cells (BMSCs)]. The SCI was induced by compression for 2 min at T10 and injury bilaterally. The femoral and tibial bones were used for bone marrow isolation and culture was made using Dulbecco’s Modified Eagle’s Medium supplemented with fetal bovine serum, L-glutamine and penicillin/streptomycin. Cell morphology was evaluated in all passages. Characterization of BMSCs was conducted by reverse transcription polymerase chain reaction and by osteogenic differentiation of BMSCs. The ELISA was undertaken for TNF-α. Open field locomotion was evaluated by Toyama mouse score. The BMSCs were plastic adherent and fibroblastic spindle-shape. MSCs were positive for CD90 and negative for CD34 and CD45. Osteogenic differentiation was noticed when stained with alizarin red. The serum TNF-α level increased after 24 h, 3 and 5 weeks post-SCI and was time dependent. The neurological score significantly improved after 8 weeks after BMSC transplantation. Transplantation of BMSCs was shown to decrease the TNF-α level and inflammation in injured spinal cord and improve the neurological outcome. These findings can be added to the literature for reduction of inflammation in SCI and improvement of neurological outcome after transplantation of BMSCs.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Noushin Gashmardi, Davood Mehrabani, Zahra Khodabandeh and Seyed Mojtaba Hosseini, 2016. Effect of Bone Marrow-derived Mesenchymal Stem Cells on Changes of Serum Levels of TNF-α and Locomotor Function after Spinal Cord Injury in Mice. Journal of Medical Sciences, 16: 16-24.

DOI: 10.3923/jms.2016.16.24

URL: https://scialert.net/abstract/?doi=jms.2016.16.24

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom