Abstract:
Cancer and some other mutation-related diseases are still remained among the
most difficult ones of clinical aspects. Regards to the mention issue attention
is drawn to dietary anti-mutations as cancer preventative agents. The presence
of anti-mutagenic agents in medicinal plants such as Zataria multiflora,
Achillea wilhelmsii and Camellia sinensiscan be considered due
to their antioxidants properties. So, in the present work, different fractions
of these plants were assessed for their anti-mutagenic effects using Ames method.
Aerial parts of the tested plants after collection, drying and milling were
extracted using petroleum ether, chloroform and 80% methanol consecutively by
percolation method. The dried extracts under vacuum were examined for their
anti-mutagenic effects. The minimum inhibition concentration (MIC) of the extracts
was evaluated by ager dilution method. Various concentrations under their measured
MIC were used for anti-mutagenic test. Each extract along with bacterial strain
and mutagen agent were incubated at 37°C for 48 h. The number of revertant
colonies was counted and compared with control plates. Our results showed that
all fractions of Z. multiflora exhibited anti-mutagenicity effect in
the presence of TA98, while only its methanolic fraction was active against
TA100. Methanolic and chloroform fractions of C. Sinensis showed strong
anti mutagenicity against TA98 only, while the methanolic fraction of A.
wilhelmsii showed anti-mutagenicity effect in the presence of both TA98
and TA100. Considering the presence of flavonoids in methanolic fraction and
high antimutagenicity effect of this fraction, flavonoids are probably responsible
for anti-mutagenic effect of the plants.
Gholamreza Dehghan-Noodeh, Fariba Sharififar, Mohammad Hassan Moshafi, Effat Behravan, Ali Dehghan-Noodeh and Reza Rezaei-Gharaeei, 2013. Antimutagenicity Activity of Different Fractions of Zataria multiflora,
Achillea wilhelmsii and Camellia sinensis using Ames Test. Journal of Medical Sciences, 13: 459-464.
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