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Journal of Medical Sciences
  Year: 2013 | Volume: 13 | Issue: 4 | Page No.: 261-268
DOI: 10.3923/jms.2013.261.268
 
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Genetic Linkage of the Antibiotic Resistance Ability in the Escherichia coli UR4 Strain Isolated from Urine

Gamal Enan, Seham Abdel-Shafi, Sahar M. Ouda and Ibrahim El-Balat

Abstract:
It is of interest to understand the antibiotic resistance ability in pathogenic bacteria at the molecular level. Characterization of bacteria by methods based on phenotypic, biochemical and molecular characters were followed herein; and plasmids were certain target in the present study. Seventeen bacterial isolates were isolated from urine, blood and stool samples of patients with urinary tract infections, bloody diarrhea and fever. They were characterized by phenotypic and biochemical criteria and were identified as belonging to Escherichia coli (E. coli). One of them, isolate number 4 from urine (UR4) was resistant to 14 types of antibiotics used. Hence this strain was subjected to molecular identification. DNA was isolated from E. coli UR4 and 16SrRNA gene was separated after agarose gel electrophoresis and then was sequenced. The sequence was subjected to gene bank and showed about 99.5% similarity to E. coli category. Growing of E. coli UR4 at elevated temperature (42°C) and treatment of colonies with sodium dodycyl sulphate, sodium azide and ethidium bromide revealed a mutants lacking antibiotic resistance ability with mutation percentage ranging from 0.6-14%. The mutation in E. coli UR4 was a stable character and no recovery of mutants was observed. Plasmid profile of the E. coli UR4 wild strain and its four mutants showed five plasmids in the wild strain and four only in its mutants. One plasmid of a molecular mass of about 600 bp is showed to be deficient or dissociated indicating on its role in antibiotic resistance ability in the E. coli UR4 strain.
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How to cite this article:

Gamal Enan, Seham Abdel-Shafi, Sahar M. Ouda and Ibrahim El-Balat, 2013. Genetic Linkage of the Antibiotic Resistance Ability in the Escherichia coli UR4 Strain Isolated from Urine. Journal of Medical Sciences, 13: 261-268.

DOI: 10.3923/jms.2013.261.268

URL: https://scialert.net/abstract/?doi=jms.2013.261.268

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