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Journal of Medical Sciences

Year: 2008 | Volume: 8 | Issue: 1 | Page No.: 54-61
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Authors

Ismaeel Bin-Jaliah

Keywords

Research Article

Comparison of Glutathione Peroxidase Activity and Free Radicals Production in the Lungs and the Brain of Rats During Graded Hyperoxia

Ismaeel Bin-Jaliah
The purpose of this study was to examine the behavior of glutathione peroxidase (GPx) activities and Free Radicals (FR) production in the brain and the lung during graded hyperoxia exposure. Twenty-four adult male rats, matched with age and body weigh, were randomly assigned to four groups. The first group served as control and the second, third and fourth were exposed to hyperoxia for 24, 48 and 72 h, respectively. Following the exposure period for each group animals were sacrificed and both lungs and brain tissues were homogenized for GPx and FR determinations. GPx activity was determined by Randox protocol (Randox, UK) and FR was determined using dROM method (H and D, Italy). Results showed that mean ( ± SD) GPx activity in the lungs increased from the baseline control of 12898.33 ± 6034.77 to 20083.62 ± 2734 (U L-1) during hyperoxia exposure for 24; then dropped to 5467.77 ± 1159.53 and 8271.80 ± 1347.67 (U L-1) during hyperoxia exposure for 48 and 72 h, respectively. Whereas mean ( ± SD) GPx activity in the brain increased from the baseline control of 5467.80 ± 2852.65 to 13841.72 ± 1245.67 and 14594.82 ± 6711.44 (U L-1), during hyperoxia exposure for 24 and 48 h, respectively; then dropped to 4346.17 ± 343.34 (U L-1), during 72 h exposure. The sustained increased in GPx up-to 48 hr in the brain provided evidence of delayed protection against ROS. The average ( ± SD) FR production in the lung increased from the baseline control of 176.67 ± 33.79 to 274.33 ± 33.37, 260.00 ± 62.54 and 320.00 ± 114.91 (U L-1) during hyperoxia exposure for 24, 48 and 72 h, respectively. The average ( ± SD) FR production in the brain increased from the baseline control of 73.33 ± 20.18 to 132.17 ± 21.77 during hyperoxia exposure for 24 h and then dropped to 94.33 ± 14.56 and 65.33 ± 21.12, during 48 and 72 h, respectively. Tukey-Kramer multiple comparisons between the lung and the brain showed that the lungs had higher rate of FR formation at all levels of hyperoxia exposures, which suggest more mechanisms that had contributed to FR formation in pnueumocyte, as compared with neurocyte. Based on the results of the present study antioxidant supplements are recommended for traumatic brain injury and hypoxemia lung injury patients subjected to oxygen therapy.
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