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Comparison of Glutathione Peroxidase Activity and Free Radicals Production in the Lungs and the Brain of Rats During Graded Hyperoxia |
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Ismaeel Bin-Jaliah |
Abstract:
The purpose of this study was to examine the behavior of glutathione
peroxidase (GPx) activities and Free Radicals (FR) production in the brain
and the lung during graded hyperoxia exposure. Twenty-four adult male
rats, matched with age and body weigh, were randomly assigned to four
groups. The first group served as control and the second, third and fourth
were exposed to hyperoxia for 24, 48 and 72 h, respectively. Following
the exposure period for each group animals were sacrificed and both lungs
and brain tissues were homogenized for GPx and FR determinations. GPx
activity was determined by Randox protocol (Randox, UK) and FR was determined
using dROM method (H and D, Italy). Results showed that mean ( ±
SD) GPx activity in the lungs increased from the baseline control of 12898.33
± 6034.77 to 20083.62 ± 2734 (U L-1) during hyperoxia
exposure for 24; then dropped to 5467.77 ± 1159.53 and 8271.80
± 1347.67 (U L-1) during hyperoxia exposure for 48 and
72 h, respectively. Whereas mean ( ± SD) GPx activity in the brain
increased from the baseline control of 5467.80 ± 2852.65 to 13841.72
± 1245.67 and 14594.82 ± 6711.44 (U L-1), during
hyperoxia exposure for 24 and 48 h, respectively; then dropped to 4346.17
± 343.34 (U L-1), during 72 h exposure. The sustained
increased in GPx up-to 48 hr in the brain provided evidence of delayed
protection against ROS. The average ( ± SD) FR production in the
lung increased from the baseline control of 176.67 ± 33.79 to 274.33
± 33.37, 260.00 ± 62.54 and 320.00 ± 114.91 (U L-1)
during hyperoxia exposure for 24, 48 and 72 h, respectively. The average
( ± SD) FR production in the brain increased from the baseline
control of 73.33 ± 20.18 to 132.17 ± 21.77 during hyperoxia
exposure for 24 h and then dropped to 94.33 ± 14.56 and 65.33 ±
21.12, during 48 and 72 h, respectively. Tukey-Kramer multiple comparisons
between the lung and the brain showed that the lungs had higher rate of
FR formation at all levels of hyperoxia exposures, which suggest more
mechanisms that had contributed to FR formation in pnueumocyte, as compared
with neurocyte. Based on the results of the present study antioxidant
supplements are recommended for traumatic brain injury and hypoxemia lung
injury patients subjected to oxygen therapy.
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How to cite this article:
Ismaeel Bin-Jaliah , 2008. Comparison of Glutathione Peroxidase Activity and Free Radicals Production in the Lungs and the Brain of Rats During Graded Hyperoxia. Journal of Medical Sciences, 8: 54-61.
DOI: 10.3923/jms.2008.54.61
URL: https://scialert.net/abstract/?doi=jms.2008.54.61
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