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Journal of Medical Sciences
  Year: 2007 | Volume: 7 | Issue: 2 | Page No.: 195-202
DOI: 10.3923/jms.2007.195.202
 
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Purification of Beta Bungarotoxin (β-Bgtx) Binding Protein from Human Cadaver Skeletal Muscle
Suhail Rasool, B.L. Jailkhani, M. Irshad, Madhuri Behari, Shahnaz Suhail and H. Shabirul

Abstract:
High affinity β-bungarotoxin binding protein was purified from skeletal muscle of human cadaver by affinity chromatography using β-bungarotoxin as a ligand. The SDS-PAGE of purified protein revealed two major protein bands with molecular weight of 86 and 68 kD. The purified protein has a kD of 2.3±0.15 nmoles and binding sites of 34±2.3 f mole/mg tissue. Immunoreactivity profile of purified presynaptic receptor (β-bungarotoxin binding protein) from muscle with myasthenic sera was negatively affected by treatment with sodium-metaperiodate, glucosidase and trypsin, where as no effect was seen on treatment with lipase. This provides evidence that purified presynaptic receptor (β-bungarotoxin binding protein) is a glycoprotein.
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How to cite this article:

Suhail Rasool, B.L. Jailkhani, M. Irshad, Madhuri Behari, Shahnaz Suhail and H. Shabirul, 2007. Purification of Beta Bungarotoxin (β-Bgtx) Binding Protein from Human Cadaver Skeletal Muscle. Journal of Medical Sciences, 7: 195-202.

DOI: 10.3923/jms.2007.195.202

URL: https://scialert.net/abstract/?doi=jms.2007.195.202

 
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