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Journal of Medical Sciences
  Year: 2007 | Volume: 7 | Issue: 1 | Page No.: 150-153
DOI: 10.3923/jms.2007.150.153
 
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Study of Betalactamase Production in Coagulase Positive Staphylococci by Iodometric and Acidometric Methods and Their Antibiotic Resistance Pattern

M. Bokaeian and M.I. Qureshi

Abstract:
Due to importance of coagulase positive staphylococci in pyogenic infections and their increasing rate of resistance to betalactam antibiotics and also important role of betalactamase enzyme in this phenomenon, we accomplished this study for isolation, mesurement of betalactamase enzyme and antibiotic resistance assay by standard methods. Total of 603 different samples obtained from patients with staphylococcal infections were cultured on general and selective media and after 24-48 h incubation at 37°C, coagulase reaction and biochemical tests were used to identification. Betalactamase assays accomplished by acidometric and idometric methods and antibiotic susceptibility of isolated strains were done by standard agar diffusion of Kirby Bauer method. Results revealed out of 603 samples, 38 coagulase positive staphylococci were isolated (6.3%). Betalactamase assay by acidometric and idometric methods were positive in 78.9 and 73.6%, respectively. All of the bacteria were resistant to carbenicillin, ampicillin and amoxycillin but on other hand resistance of other betalactams include as: penicillin (97.3%), oxacillin and methicillin (18.4%), ceftizoxime (17%), cephalothin (15.7%), ceftriaxon (14%), cephalexin (13.1%), cefazolin (7.8%). In accordance with high resistance of isolated coagulase positive staphylococci to carbenicillin, penicillin, ampicillin and amoxycillin and high rate of betalactamase production in these pathogen bacteria that lead to resistance to betalactam antibiotics we suggest the attention of physicians to the role of the bacteria and their proper treatment in the city.
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How to cite this article:

M. Bokaeian and M.I. Qureshi, 2007. Study of Betalactamase Production in Coagulase Positive Staphylococci by Iodometric and Acidometric Methods and Their Antibiotic Resistance Pattern. Journal of Medical Sciences, 7: 150-153.

DOI: 10.3923/jms.2007.150.153

URL: https://scialert.net/abstract/?doi=jms.2007.150.153

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