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Journal of Medical Sciences
  Year: 2006 | Volume: 6 | Issue: 3 | Page No.: 420-425
DOI: 10.3923/jms.2006.420.425
 
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Antimitotic Effect of Colchicine from Six Different Species of Gloriosa in Onion Roots (Allium cepa)
P. Bharathi, D. Philomina and S. Chakkaravarthi

Abstract:
Colchicine, a chief alkaloid was determined in six different species of Gloriosa. Solvent extraction of colchicine with petroleum ether and dichlomethane and quantification through High Performance Liquid Chromatography showed high level of colchicine (0.342 mg g -1) in Gloriosa planti amongst the species selected for the study. The mitotic inhibition of colchicine in onion root was standardized using standard colchicine. The effect of colchicine extracted from Gloriosa species was studied in onion root tips treated with 30 mg L -1 colchicine for 2 h. Mitotic abnormalities have been observed and reported for the extracts from different species of Gloriosa under study.
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How to cite this article:

P. Bharathi, D. Philomina and S. Chakkaravarthi , 2006. Antimitotic Effect of Colchicine from Six Different Species of Gloriosa in Onion Roots (Allium cepa). Journal of Medical Sciences, 6: 420-425.

DOI: 10.3923/jms.2006.420.425

URL: https://scialert.net/abstract/?doi=jms.2006.420.425

COMMENTS
15 May, 2009
SOUMANA DATTA:
how was the colchicine applied? were the root tips kept in the soln, or you used the dropper method?

can we do this expt using allium cepa root tips? would love to know more about the protocol used.

thanks
 
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