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Journal of Medical Sciences
  Year: 2005 | Volume: 5 | Issue: 3 | Page No.: 169-176
DOI: 10.3923/jms.2005.169.176
 
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Altered Proliferative Response of Normal and Malignant Cells Exposed to a Polysaccharide Extract from Tamarindus indica Pulp

Fernando Garci Tamayo, Teresa Izquierdo , Guadalupe Reyes , Patricia Elizalde , Elba Rojas , Emilio Rojas del Castillo and Luis I. Terrazas

Abstract:
A water-soluble Polysaccharide Extract (PE) isolated from the pulp of the fruit bodies of a Mexican species of Tamarindus indica L. was characterized by thin-layer chromatography and HPLC. Fructose, glucose, arabinose and xylose were the major components of the hydrolyzed PE samples. Normal lymphocytes and several lineages of malignant cells were cultured in presence of different amounts of PE solution and their effects on cellular proliferation were assessed by tritiated thymidine uptake. After incubation for 48 h, a significant reduction on the thymidine uptake was observed when Con-A-stimulated mice’s splenocytes were incubated with tamarind PE. Furthermore, a similar reduction in proliferative activity was detected also in CTLL-2 and HeLa cell lines. A comparative control assay showed a more intense inhibition when similar amounts (5-100 μg mL-1) of cis-platinum was added to HeLa cells. The morphological examination of PE-treated HeLa cells revealed their transformation into multinucleated giant cells. The results suggest that polysaccharide extracts obtained from tamarind pulp may contain active inhibitors of in vitro cellular multiplication.
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How to cite this article:

Fernando Garci Tamayo, Teresa Izquierdo , Guadalupe Reyes , Patricia Elizalde , Elba Rojas , Emilio Rojas del Castillo and Luis I. Terrazas , 2005. Altered Proliferative Response of Normal and Malignant Cells Exposed to a Polysaccharide Extract from Tamarindus indica Pulp. Journal of Medical Sciences, 5: 169-176.

DOI: 10.3923/jms.2005.169.176

URL: https://scialert.net/abstract/?doi=jms.2005.169.176

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