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Journal of Medical Sciences
  Year: 2002 | Volume: 2 | Issue: 1 | Page No.: 19-23
DOI: 10.3923/jms.2002.19.23
 
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Effect of Bisphenol-A on the Sertoli Cell Culture from Prepubertal Male Wistar Rats

Mohammad A. Awal, Masamichi Kurohmaru , Takuo Mizukami , Bibin B. Andriana , Yoshiakira Kanai and Yoshihiro Hayashi

Abstract:
The investigation was conducted to evaluate the effect of bisphenol-A on the sertoli cell culture from prepubertal Wistar rats. Sertoli cell culture was obtained by sequential collagenase and trypsin-EDTA treatment. The cell culture was incubated and maintained for 21 days. The culture was trypsinized and seeded at an appropriate density. Three days later, bisphenol-A was added to the cell culture at the concentration of 10, 100 and 1000pg ml-1, respectively. Control group was treated with saline. The cultured cells were examined by light and transmission electron microscopy. A phase contrast microscope was also used to observe the morphological changes of the cells. At 2 days after treatment, the increase of degenerative sertoli cell was 7.2, 14.7, and 20.8% at the concentration of 10, 100, and 1000pg ml-1, respectively. Similarly, at 4 days, those increased as 11.4, 19.5, 28.3%, and at 6 days as 12.6, 21.3 and 34.9%, respectively. The maximum degenerative change of sertoli cell was observed at the concentration of 1000pg ml-1 and at 6 days after treatment. They were significantly different from those in the control group (p<0.05). Distinct histopathological changes in the sertoli cell culture were noted after exposure to bisphenol-A. This findings suggested that bisphenol-A showed a direct effect on sertoli cells in culture.
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How to cite this article:

Mohammad A. Awal, Masamichi Kurohmaru , Takuo Mizukami , Bibin B. Andriana , Yoshiakira Kanai and Yoshihiro Hayashi , 2002. Effect of Bisphenol-A on the Sertoli Cell Culture from Prepubertal Male Wistar Rats. Journal of Medical Sciences, 2: 19-23.

DOI: 10.3923/jms.2002.19.23

URL: https://scialert.net/abstract/?doi=jms.2002.19.23

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