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Effect of Bisphenol-A on the Sertoli Cell Culture from Prepubertal Male Wistar Rats |
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Mohammad A. Awal,
Masamichi Kurohmaru ,
Takuo Mizukami ,
Bibin B. Andriana ,
Yoshiakira Kanai and Yoshihiro Hayashi |
Abstract:
The investigation was conducted to evaluate the effect of bisphenol-A on the
sertoli cell culture from prepubertal Wistar rats. Sertoli cell culture was obtained
by sequential collagenase and trypsin-EDTA treatment. The cell culture was
incubated and maintained for 21 days. The culture was trypsinized and seeded at
an appropriate density. Three days later, bisphenol-A was added to the cell culture
at the concentration of 10, 100 and 1000pg ml-1, respectively. Control group was
treated with saline. The cultured cells were examined by light and transmission
electron microscopy. A phase contrast microscope was also used to observe the
morphological changes of the cells. At 2 days after treatment, the increase of
degenerative sertoli cell was 7.2, 14.7, and 20.8% at the concentration of 10, 100,
and 1000pg ml-1, respectively. Similarly, at 4 days, those increased as 11.4, 19.5,
28.3%, and at 6 days as 12.6, 21.3 and 34.9%, respectively. The maximum
degenerative change of sertoli cell was observed at the concentration of 1000pg
ml-1 and at 6 days after treatment. They were significantly different from those in
the control group (p<0.05). Distinct histopathological changes in the sertoli cell
culture were noted after exposure to bisphenol-A. This findings suggested that
bisphenol-A showed a direct effect on sertoli cells in culture.
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How to cite this article:
Mohammad A. Awal, Masamichi Kurohmaru , Takuo Mizukami , Bibin B. Andriana , Yoshiakira Kanai and Yoshihiro Hayashi , 2002. Effect of Bisphenol-A on the Sertoli Cell Culture from Prepubertal Male Wistar Rats. Journal of Medical Sciences, 2: 19-23.
DOI: 10.3923/jms.2002.19.23
URL: https://scialert.net/abstract/?doi=jms.2002.19.23
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