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Journal of Medical Sciences
  Year: 2002 | Volume: 2 | Issue: 1 | Page No.: 12-18
DOI: 10.3923/jms.2002.12.18
 
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Primary Physiological Effect of the Sub-Inhibitory Concentrations of Formalin, TH4+ and Virkon-S on Escherichia coli Serotype O55: K39
Moustafa Y. El-Naggar, Mohamed A. Akeila , Helmi. A. Turk, Ali. A. El-Ebady and Mona. Z. Sahaly

Abstract:
The primary physiological effect of TH4+ and Virkon-S on E. coli serotype O55: K39 cells in comparison to formalin was investigated. The in vitro activity of the disinfectants indicated that TH4+ and Virkon-S caused a leakage of sodium (Na), potassium (K) ions and UV-absorbing materials. The leakage level was directly proportional to disinfectant concentration and to the exposure time. Virkon-S recorded higher efflux of Na+ and K+ ions than TH4+. The X-ray microanalysis for E. coli cells treated with Virkon-S recorded a low elemental level for P, S, K, Na, Cu, Zn and Mg. TH4+ caused a lowering in Na, K, S, Cl, Fe and P levels, whereas Cu and Zn levels were similar to the control. On the other hand, TH4+ showed a superior effect on the leakage of UV-absorbing material compared to Virkon-S. The amount of total protein of E. coli serotype O55: K39 decreased with the increase of disinfectant concentration. Virkon-S recorded the highest inhibition for protein synthesis, followed by formalin compared to TH4+. The electrophoretic profiles demonstrated a considerable difference in the protein pattern of the treated cells as compared to the control. Formalin treated cells (0.015 and 0.007%) revealed that the absence of most of the protein bands between 18.5-36 kDa and all bands above 36 kDa. Virkon-S treated cells (at 0.015%) masked the bands at 36-330 kDa, whereas Virkon-S (0.007%) and TH4+ (0.007 and 0.015%) addition showed a typical protein banding which matched that of the control.
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How to cite this article:

Moustafa Y. El-Naggar, Mohamed A. Akeila , Helmi. A. Turk, Ali. A. El-Ebady and Mona. Z. Sahaly , 2002. Primary Physiological Effect of the Sub-Inhibitory Concentrations of Formalin, TH4+ and Virkon-S on Escherichia coli Serotype O55: K39. Journal of Medical Sciences, 2: 12-18.

DOI: 10.3923/jms.2002.12.18

URL: https://scialert.net/abstract/?doi=jms.2002.12.18

 
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