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Research Journal of Microbiology
  Year: 2021 | Volume: 16 | Issue: 1 | Page No.: 8-18
DOI: 10.3923/jm.2021.8.18
 
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Potential Antimicrobial Substances from the Characterized Bioactive Compounds Extracted from Secondary Metabolites of Aspergillus terreus

Kelechi Oleru, Afolake Olanbiwoninu, John Olayiwola and Bukola Popoola

Abstract:
Background and Objective: The menace of MDR bacteria and search for the potent antimicrobial substance remain a relevant research. Fungi are commonly recognized as microorganisms that play role in the production of secondary metabolites which are antimicrobials. Therefore, this study focused on the antimicrobial properties of the fungal secondary metabolites and their bioactive compounds. Materials and Methods: Soil samples were collected from the rhizosphere region of different farm gardens in Oyo town, Nigeria. Isolation of the fungi was carried out using Potato Dextrose agar, identified through the amplification of the ITS region of the ribosomal RNA operon and identified to be Aspergillus terreus. The isolates were screened for the production of secondary metabolites by batch culture using an incubator shaker at 27°C for 5 days. The metabolite was extracted and concentrated using a rotary evaporator and aliquots of the metabolites were stored at 4°C. Agar-well diffusion assay was employed to evaluate the antibacterial properties of the secondary metabolites of the fungus. The bioactive chemical compounds of the metabolite extracted from fungus were evaluated using the GC-MS technique. Results: The bacterial pathogens investigated in this work were multi-drug resistant bacteria with a minimum resistant rate of 61%. The MDR bacterial pathogens were all susceptible to the secondary metabolite of Aspergillus terreus in this study. Conclusion: The bioactive compounds evaluated in this work showed the occurrence of organic compounds in the metabolite therefore secondary metabolite of Aspergillus terreus holds the chance of discovering the novel and potent drug.
Citation
How to cite this article:

Kelechi Oleru, Afolake Olanbiwoninu, John Olayiwola and Bukola Popoola, 2021. Potential Antimicrobial Substances from the Characterized Bioactive Compounds Extracted from Secondary Metabolites of Aspergillus terreus. Research Journal of Microbiology, 16: 8-18.

DOI: 10.3923/jm.2021.8.18

URL: https://scialert.net/abstract/?doi=jm.2021.8.18

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