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Research Journal of Microbiology
  Year: 2021 | Volume: 16 | Issue: 1 | Page No.: 1-7
DOI: 10.3923/jm.2021.1.7
 
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Quantitation of mecA and sea genes on Staphylococcus aureus using Quantitative PCR Assay

S.K.S. Ojo , F.I. Esumeh and G.T. Sunmonu

Abstract:
Background and Objective: Methicillin Resistant Staphylococcus aureus (MRSA) is one of the major causes of nosocomial infections and are most profound in community in previously healthy individuals. To detect and quantify antibiotic resistant and virulence genes present in methicillin sensitive S. aureus (MSSA) strains from wounds and burns patients. Materials and Methods: About 200 clinical samples were obtained for S. aureus isolation, identified and characterized by using standard microbiological procedures. Methicillin resistance was determined by using β-lactamase assay and oxacillin disk (Oxoid) susceptibility test. Quantification of the S. aureus strains was performed using quantitative Polymerase Chain Reaction (qPCR) assay. Agarose gel electrophoresis was carried out on the qPCR products using 1.5% agarose gel with a standard DNA ladder (100 bp), visualized under UV transilluminator and the image taken using digital camera. Results: Almost 44 (22%) S. aureus were isolated and characterized with 36 (82%) strains producing β-lactamase and were resistant to oxacillin (MRSA) while, 8 (18%) strains do not produce β-lactamase and were sensitive to oxacillin (MSSA). The β-lactamase and non-β-lactamase isolates were resistant to other antibiotics. The quantification of PCR products indicated that sea genes (virulence enterotoxin factor) were detected from the antibiotic resistant staphylococci ranging from 0-13551.84 nmoles while, the quantification of mecA genes detected ranged from 0-2601.76 nmoles. The agarose gel electrophoresis of the PCR products of mecA and sea genes showed amplicon size of 657 bp for mecA and 526 bp for sea genes after amplification of the antibiotic resistant S. aureus strains. Conclusion: This study detected the presence of antibiotic resistant and virulence genes associated with MRSA in MSSA, which calls for urgent clinical and pharmaceutical attention.
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How to cite this article:

S.K.S. Ojo, F.I. Esumeh and G.T. Sunmonu, 2021. Quantitation of mecA and sea genes on Staphylococcus aureus using Quantitative PCR Assay. Research Journal of Microbiology, 16: 1-7.

DOI: 10.3923/jm.2021.1.7

URL: https://scialert.net/abstract/?doi=jm.2021.1.7

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