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Research Journal of Microbiology
  Year: 2019 | Volume: 14 | Issue: 1 | Page No.: 15-19
DOI: 10.3923/jm.2019.15.19
 
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First Documented Mastitis Case of Nocardia puris in Turkey from Two Cows: Microbiological and Molecular Identification
Ozlem Sahan Yapicier , Dilek Ozturk and Mehmet Kaya

Abstract:
Background and Objective: Nocardia is one of the causing agents of bovine mastitis and increasing prevalence of nocardial mastitis from many countries. The aim of the present study was to characterize Nocardia puris isolated from two bovine mastitis cases in Turkey based on microbiological and molecular methods. Materials and Methods: Nocardia sp. was isolated from milk samples from all quarters of 1 Simmental and 1 Holstein cows submitted to Mehmet Akif Ersoy University Faculty of Veterinary Medicine, Department of Microbiology laboratory in Burdur on April, 2018 and confirmed by 16S rRNA gene sequencing. Antimicrobial resistance was assessed by disc diffusion against ampicillin, cefoperazone, ceftiofur, cephalexin cloxacillin, enrofloxacin, gentamicin, oxytetracycline, penicillin and trimethoprim sulphamethoxazole. Results: A total of 3 Nocardia spp. were isolated and molecular analysis of the obtained 16S rRNA gene sequence of 3 Nocardia isolates with BLAST revealed that the isolates had a 99% sequence similarity with N. puris strains. Based on the disc diffusion test, all N. puris isolates were sensitive to gentamicin, enrofloxacin, ceftiofur, oxytetracycline and sulfamethoxazole trimethoprim and resistance to ampicillin, cefoperazone, cloxacillin, cephalexin and penicillin. Conclusion: Overall, current results suggested that a well-defined mastitis etiology and this is apparently the first report of N. puris in association with bovine mastitis in Turkey.
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How to cite this article:

Ozlem Sahan Yapicier, Dilek Ozturk and Mehmet Kaya, 2019. First Documented Mastitis Case of Nocardia puris in Turkey from Two Cows: Microbiological and Molecular Identification. Research Journal of Microbiology, 14: 15-19.

DOI: 10.3923/jm.2019.15.19

URL: https://scialert.net/abstract/?doi=jm.2019.15.19

 
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