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Research Journal of Microbiology
  Year: 2018 | Volume: 13 | Issue: 2 | Page No.: 84-92
 
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Prevalence and Genotypes of Rotavirus Infection among Children with Gastroenteritis in Abuja, Nigeria

Theophilus Kachi Udeani , Uzoamaka Chukwuma Ohiri, Okechukwu Steven Onwukwe and Chukwubike Chinedu

Abstract:
Background and Objectives: Rotavirus is a major cause of severe diarrhea in children worldwide. The existing genotypes of rotavirus in Nigeria are not widely known. This cross-sectional study investigated the prevalence, risk factors for rotavirus antigens and circulating genotypes amongst children within 5 years of age in Abuja, Nigeria. Materials and Methods: Watery and/or semi-solid stool samples were collected from children and preserved in glycerol. A structured questionnaire was used to obtain information on demographic characteristics and clinical details from their parents. Rotavirus antigen was detected in the stool specimens by Rotavirus antigen kit, while genotype characterization for G- and P-type genotypes were by reverse transcription polymerase chain reaction and polyacrylamide gel electrophoresis. Descriptive and chi-square statistics were used to analyze all the data obtained. Results: Of 144 stool samples analyzed, 25% (36) had Rotavirus infection, with male dominance than females15.3% (22/144) and 9.7% (14/144), respectively. The age group of less than 10 months had higher rotavirus infection of 13.9% (20/144). The major statistical significant risk factors identified were, use of feeding bottles, p = 0.003 and attendance to day care centers, p = 0.038. The peak of rotavirus infection was April, with a range of January-May. The prevalent genotypes identified were G1, G3, G9, G12, (VP7) and P8, P6 (VP4) with mixed G/P. The G12 genotype was the predominant G-type. The migration pattern of the viral genome showed that the long genomic segments were more than the short ones. Conclusion: The G12 genotypes were highly associated with rotavirus-diarrhea and disease amongst the children. There is a need to introduce rotavirus vaccination in the National program on Immunization in Nigeria.
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How to cite this article:

Theophilus Kachi Udeani, Uzoamaka Chukwuma Ohiri, Okechukwu Steven Onwukwe and Chukwubike Chinedu, 2018. Prevalence and Genotypes of Rotavirus Infection among Children with Gastroenteritis in Abuja, Nigeria. Research Journal of Microbiology, 13: 84-92.

URL: https://scialert.net/abstract/?doi=jm.2018.84.92

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