Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Research Journal of Microbiology
  Year: 2018 | Volume: 13 | Issue: 1 | Page No.: 1-12
DOI: 10.3923/jm.2018.1.12
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Biofilm Formation and its Susceptibility Testing of Myroides odoratimimus SKS05-GRD Using MBECTM High-throughput Assay
Mohanapriya Madheswaran, Saranya Asokan, Nitya Meenakshi Raman, Nidhiya Anil Kumar and Suganthi Ramasamy

Abstract:
Background and Objective: Bacterial biofilms are highly regarded for their increasing potential to resist antibiotics and cause infections especially on food and beverage based substrates. These bacteria with a potential to develop biofilms are now researched with the hindsight to control the contamination they can cause. In this study the biofilm forming ability of Myroides odoratimimus (M. odoratimimus) SKS05-GRD has been evaluated using different substrates and different time intervals. Materials and Methods: Determination of minimal inhibitory concentration (MIC) and minimal biofilm eradication concentration (MBEC) of each antimicrobial agents was compared to test efficacy of the antibiotics and nanoparticles. Bioanalytical instrumentation such as scanning electron microscopy (SEM) and Fourier Transform Infra Red (FT-IR) spectroscopic analysis and statistical validation (ANOVA) on the pre-formed biofilm was carried out. Results: Growth curve analysis revealed an exponential phase at 47 min while optimum biofilm forming potential was observed at 72 h. Parametric evaluations of media and substrate revealed food flavobacterium medium and plastic was favoured by the bacterium for optimum biofilm formation. Although, silver nanoparticles by themselves render antibiofilm activity, results of MIC, MBEC, SEM and FT-IR analysis suggest enhanced reduction in biofilm formation when the nanoparticles combined with tetracycline. Conclusion: This study confirms the potential of Myroides odoratimimus SKS05-GRD for biofilm formation and the eradication of the same using tetracycline complexed with silver nanoparticles.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Mohanapriya Madheswaran, Saranya Asokan, Nitya Meenakshi Raman, Nidhiya Anil Kumar and Suganthi Ramasamy, 2018. Biofilm Formation and its Susceptibility Testing of Myroides odoratimimus SKS05-GRD Using MBECTM High-throughput Assay. Research Journal of Microbiology, 13: 1-12.

DOI: 10.3923/jm.2018.1.12

URL: https://scialert.net/abstract/?doi=jm.2018.1.12

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

       

       

Curve Bottom