Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Research Journal of Microbiology
  Year: 2017 | Volume: 12 | Issue: 4 | Page No.: 229-235
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Reutilization of Microbial Cells for Production of Cyclodextrin Glycosyltransferase Enzyme

Natalia Sozza Bernardi, Kate Cristina Blanco and Jonas Contiero

Abstract:
Background and Objective: Cell immobilization methods have been developed in order to improve enzymatic production. The aim of this work was to use immobilized bacteria on enzyme production to improve cell stability and to enhance the time of fermentation. Materials and Methods: The variables sodium alginate, number of cells, time of gelation, aeration and silica concentration in cells immobilization for CGTase production were evaluated. Statistical analysis of enzymatic response was performed using paired t-test (0.5%). Results: The better conditions for maximal production of CGTase (389.94 U mL–1) with immobilization of Bacillus circulans ATCC 21783 were: 3% sodium alginate; 0.2 g L–1 initial cells; 100 rpm aeration and 3% silica. There was an increase of 83.05% in enzymatic production with cell entrapment in silica. The beads cell reutilization was possible during three cycles of fermentation during 216 h. Conclusion: The enzymatic production with the beads cells reutilization provided an increase in productivity and low costs of fermentation processes. The immobilization of cells in silica beads maintains cell stability and allows the reuse of the system during CGTase production.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Natalia Sozza Bernardi, Kate Cristina Blanco and Jonas Contiero, 2017. Reutilization of Microbial Cells for Production of Cyclodextrin Glycosyltransferase Enzyme. Research Journal of Microbiology, 12: 229-235.

URL: https://scialert.net/abstract/?doi=jm.2017.229.235

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
Curve Bottom