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Research Journal of Microbiology
  Year: 2015 | Volume: 10 | Issue: 6 | Page No.: 246-259
 
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Potency of Auxin Producing and Phosphate Solubilizing Bacteria from Dryland in Rice Paddy Field

Gergonius Fallo, Nisa Rachmania Mubarik and Triadiati

Abstract:
Rice (Oryza sativa L.) is one of important and suitable corps in East Indonesia including North Central Timor (NCT) Regency. However, farmer NCT Regency have not yet use microbe as biofertilizer. The research aimed to isolate Indole Acetic Acid (IAA) producing by soil bacteria from paddy field in NCT Regency and to apply selected microbe in rice cultivation. Eight isolates were obtained and analyzed their IAA ability based on a colorimetric method using Salkowski reagent. The result showed that the producing eight isolates was able to synthesize IAA with the highest concentration produced by EP.01 isolate. The ability of these isolate to solubilize phosphate was measured by using Pikovskaya media. The EP.02 isolate has the highest ability to solubilize phosphate. The growth curve and IAA synthesis were created using EP.01 and EP.02 isolates as a model. Production of IAA was in line with the cells growth. Both EP.01 and EP.02 isolates were closely related to Bacillus sp. with 97.7 and 98.1% maximum identity, respectively. The application of Bacillus sp. solubilizer as biofertilizer on paddy field using randomized block design with the type of fertilizer as a single factor. Application of fertilizer used compost enriched with 50% NPK, had the best result on the number of filled grains per clump, dry weight of 1000 grains which were 30.76, 29.37 g and 6.3 t ha-1, respectively.
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How to cite this article:

Gergonius Fallo, Nisa Rachmania Mubarik and Triadiati , 2015. Potency of Auxin Producing and Phosphate Solubilizing Bacteria from Dryland in Rice Paddy Field. Research Journal of Microbiology, 10: 246-259.

URL: https://scialert.net/abstract/?doi=jm.2015.246.259

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